Nitrocefin [41906-86-9]
Cat# B6052-10mg
Size : 10mg
Marca : APExBIO Technology
Nitrocefin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Nitrocefin is a chromogenic cephalosporin substrate which is used for detection of β-lactamases. β-lactamases are enzymes produced by various microbes that mediate resistance to β-lactam antibiotics such as penicillins and cephamycins. Comparing to other methods for β-lactamases detection, nitrocefin is more sensitive while using few materials and inexpensive equipment. The color change by nitrocefin can be followed quantitatively by measuring changes in absorption between 380 to 500 nm region[1].
Reference:
[1] O'Callaghan, Cynthia H et al. Novel Method for Detection of B-Lactamases by Using a Chromogenic Cephalosporin Substrate. Antimicrobial Agents and Chemotherapy. 1972 Apr; 1(4):283-288.
- 1. Liu X, Ma L, et al. "Synergistic antibacterial mechanism of Bi(2)Te(3) nanoparticles combined with the ineffective β-lactam antibiotic cefotaxime against methicillin-resistant Staphylococcus aureus." J Inorg Biochem. 2019 Jul;196:110687." PMID:31004991
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 516.50 |
Cas No. | 41906-86-9 |
Formula | C21H16N4O8S2 |
Solubility | insoluble in EtOH; insoluble in H2O; ≥20.24 mg/mL in DMSO |
Chemical Name | (6R,7R)-3-((E)-2,4-dinitrostyryl)-8-oxo-7-(2-(thiophen-2-yl)acetamido)-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid |
SDF | Download SDF |
Canonical SMILES | 04[C@]1([C@@H]2NC(CC3=CC=CS3)=O)SCC(/C=C/C4=CC=C([N+]([O-])=O)C=C4[N+]([O-])=O)=C(C(O)=O)N1C2=O |
Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
General tips | We do not recommend long-term storage for the solution, please use it up soon. |