NKAIN4 (NM_152864) Human Tagged ORF Clone
CAT#: RC207239
NKAIN4 (Myc-DDK-tagged)-Human Na+/K+ transporting ATPase interacting 4 (NKAIN4)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | NKAIN4 |
Synonyms | bA261N11.2; C20orf58; FAM77A |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data | >RC207239 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTCCTGCTCCGGCCGCTGCGCGCTCGTCGTCCTCTGCGCTTTTCAGCTGGTCGCCGCCCTGGAGA GGCAGGTGTTTGACTTCCTGGGCTACCAGTGGGCGCCCATCCTGGCCAACTTTGTCCACATCATCATCGT CATCCTGGGACTCTTCGGCACCATCCAGTACCGGCTGCGCTATGTCATGGTGTACACGCTGTGGGCAGCC GTCTGGGTCACCTGGAACGTCTTCATCATCTGCTTCTACCTGGAAGTCGGTGGCCTCTTAAAGGACAGCG AGCTACTGACCTTCAGCCTCTCCCGGCATCGCTCCTGGTGGCGTGAGCGCTGGCCAGGCTGTCTGCATGA GGAGGTGCCAGCAGTGGGCCTCGGGGCCCCCCATGGCCAGGCCCTGGTGTCAGGTGCTGGCTGTGCCCTG GAGCCCAGCTATGTGGAGGCCCTACACAGTTGCCTGCAGATCCTGATCGCGCTTCTGGGCTTTGTCTGTG GCTGCCAGGTGGTCAGCGTGTTTACGGATGAAGAGGACAGCTTTGATTTCATTGGTGGATTTGATCCATT TCCTCTCTACCATGTCAATGAAAAGCCATCCAGTCTCTTGTCCAAGCAGGTGTACTTGCCTGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207239 protein sequence Red=Cloning site Green=Tags(s) MGSCSGRCALVVLCAFQLVAALERQVFDFLGYQWAPILANFVHIIIVILGLFGTIQYRLRYVMVYTLWAA VWVTWNVFIICFYLEVGGLLKDSELLTFSLSRHRSWWRERWPGCLHEEVPAVGLGAPHGQALVSGAGCAL EPSYVEALHSCLQILIALLGFVCGCQVVSVFTDEEDSFDFIGGFDPFPLYHVNEKPSSLLSKQVYLPA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms | CHROMATOGRAMS Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_152864 |
ORF Size | 624 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_152864.2, NP_690603.2 |
RefSeq Size | 1447 bp |
RefSeq ORF | 627 bp |
Locus ID | 128414 |
UniProt ID | Q8IVV8 |
Cytogenetics | 20q13.33 |
Protein Families | Transmembrane |
MW | 23.2 kDa |
Gene Summary | NKAIN4 is a member of a family of mammalian proteins (see NKAIN1; MIM 612871) with similarity to Drosophila Nkain and interacts with the beta subunit of Na,K-ATPase (ATP1B1; MIM 182330) (Gorokhova et al., 2007 [PubMed 17606467]).[supplied by OMIM, Jun 2009] |
Documents
Product Manuals |
FAQs |
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SDS |
Resources
Other Versions
SKU | Description | Size | |
---|---|---|---|
RC207239L3 | Lenti ORF clone of Human Na+/K+ transporting ATPase interacting 4 (NKAIN4), Myc-DDK-tagged | 600,00 EUR | |
RC207239L4 | Lenti ORF clone of Human Na+/K+ transporting ATPase interacting 4 (NKAIN4), mGFP tagged | 600,00 EUR | |
RG207239 | NKAIN4 (tGFP-tagged) - Human Na+/K+ transporting ATPase interacting 4 (NKAIN4) | 500,00 EUR | |
SC125746 | NKAIN4 (untagged)-Human Na+/K+ transporting ATPase interacting 4 (NKAIN4) | 300,00 EUR |