Immunocytochemistry Kit
| ELISA, WB, ICC |
Species Reactivity
| Hu, Rt, Ms, Ck, F |
 | KIT SUMMARY The organelle market kit can be used to co-localize proteins to specific organelles in cells. The kit includes a panel of antibodies that detect the following cell organelles: |
Immunocytochemical labeling of calnexin (CM4371) (top left), Hsp60 (HM4381) (top middle), EEA1 (EM3471) (top right), GM130 (GM3421) (bottom left), histone H2B (HP4291) (bottom middle), and caveolin-1 (CP2781) (bottom right). The antibodies were detected using secondary antibodies conjugated to DyLight® 488 or DyLight® 594.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
GM3421 Martín-Acebes, M.A. et al. (2014) J virology 88(20): 12041. Data not shownHP4291 Seo, Y. et al. (2013) PLoS ONE 8(8):e75005. WB: MDCK, A549, and HEK293
HP4291 Soe, K.C. et al. (2013) BBA 1829(11):1184. WB: Sf9 cells
GM3421 Martın-Acebes, M.A. et al. (2011) PLoS ONE 6(9): e24970. ICC: monkey Vero cell
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | ICC DILUTION |
| Calnexin (N-terminal region) Mouse mAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 1:50 | |
| Caveolin-1 (N-terminal region) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck | 1:100 | |
| Early Endosome Antigen (EEA1) Mouse mAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 1:50 | |
| GM130 (C-terminal region) Mouse mAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 1:50 | |
| Histone H2B (C-terminus)Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, F | 1:200 | |
| Hsp60 (N-terminal region) Mouse mAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 1:100 |