Penicillinase antibody (Biotin)
Cat# orb344977-100ug
Size : 100ug
Marca : Biorbyt
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Telefono : +1 850 650 7790
Penicillinase antibody (Biotin)
Catalog Number: orb344977
| Catalog Number | orb344977 |
|---|---|
| Category | Antibodies |
| Description | Penicillinase antibody (Biotin) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | DOT, IP, WB |
| Reactivity | Bacteria |
| Isotype | IgG |
| Immunogen | Penicillinase [Enterobacter cloacae] |
| Concentration | 1.0 mg/mL |
| Dilution range | IP: 1:100, WB: 1:500 - 1:2,000 |
| Form/Appearance | Lyophilized |
| Purity | Anti-Penicillinase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Penicillinase [Enterobacter cloacae]. Cross reactivity against Penicillinase from other sources is unknown. |
| Conjugation | Biotin |
| UniProt ID | P05364 |
| NCBI | AAO24600.1 |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | rabbit anti-Penicillinase Antibody biotin Conjugat |
| Note | For research use only |
| Application notes | Anti-Penicillinase Biotin Conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Penicillinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. |
| Expiration Date | 12 months from date of receipt. |
Dot Blot results of Rabbit Anti-Penicillinase Antibody Biotin Conjugated. Dots are Penicillinase at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 30 min at RT. Primary Antibody: Rabbit Anti-Penicillinase Biotin at 1 µg/mL for 1 hr at RT. Secondary Antibody: Streptavidin-HRP (p/n orb348767) at 1:40000 for 30 min at RT.