Polymyxin B (PMB) mouse monoclonal antibody, clone 45, Low Endotoxin
Specifications
Product Data
Clone Name
45
Application
ELISA, WB
Recommended Dilution
Western blotting: Use 1/50 as starting working dilution. Immunoassays.
Reactivity
Broad
Antibody Host
Mouse
Isotype
IgM
Clonality
Monoclonal
Specificity
This Monoclonal antibody clone 45 reacts with Polymyxin B. It binds to free Polymyxin B as well as to Polymyxin B already bound to LPS. This antibody enables the possibilities to study quantitatively the interaction of PMB and LPS.
Buffer
PBS with 0.1% BSA and 0.02% Sodium Azide. State: Culture Medium with a Low Endotoxin.
Concentration
lot specific
Conjugation
Unconjugated
Storage
Store the antibody undiluted at 2-8°C.
Stability
Shelf life: one year from despatch.
Shipping
Blue Ice
Background
The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region. PMB has relevance for endotoxin research in at least two ways: first, PMB reacts with LPS of many species regardless of varied serospecificity, and thus it can be used as a general probe for measuring or detecting LPS or lipid A. Second, binding of PMB to LPS may result in neutralization of the detrimental effects of LPS either in vitro or in vivo.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
