Propidium (Iodide) [25535-16-4]
Cat# HY-D0815-500mg
Size : 500mg
Marca : MedChemExpress
Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis.
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Propidium Iodide Chemical Structure
CAS No. : 25535-16-4
This product is a controlled substance and not for sale in your territory.
Based on 52 publication(s) in Google Scholar
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Propidium Iodide purchased from MedChemExpress. Usage Cited in: ACS Nano. 2023 Feb 8. [Abstract]
- Propidium iodide (PI) staining for cell death with GCFN and ferroptosis inhibitor ferrostatin-1 treatment for 48 h
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Propidium Iodide purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2022 Apr 5;920:174830. [Abstract]
- Primary newborn rat cardiac fibroblasts are stained with PI (2 μg/mL; for 2 h) and analyzed using a Nikon Ti2 microscope.
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Propidium Iodide purchased from MedChemExpress. Usage Cited in: Molecules. 2022 Oct 9;27(19):6733. [Abstract]
- In A549 cells, Propidium iodide (PI, 50 μg/mL) is added for incubation for 30 min in the dark. Cell-cycle distribution is determined using a FACSaria-I flow cytometer.
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Propidium Iodide purchased from MedChemExpress. Usage Cited in: Thorac Cancer. 2022 May;13(9):1299-1310. [Abstract]
- KYSE450 and ECA109 cells after 96 h various transfections were washed three times in cold PBS, stained with Propidium iodide (PI, 50 μg/mL) and Annexin V‐FITC (25 μg/mL) for 20 min in the dark, and analyzed within 1 h.
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Propidium Iodide purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Feb;49:102207. [Abstract]
- PAMs and PK-15CD163 cells sre washed with phosphate-buffered saline (PBS), and then incubated with the nuclear dye propidium iodide for 15 min. The nuclei sre counterstained with propidium iodide (red). Fluorescent images are acquired with a confocal laser scanning microscope.
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Propidium Iodide purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2021 Aug 31;2021:5608133.
- The NP tissue slices are incubated with a culture medium containing 1 μg/ml Calcein AM and 1 μg/ml PI for one hour at 37°C. After washing with PBS, the slices are immediately viewed using an inverted confocal laser scanning microscope.
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Propidium Iodide purchased from MedChemExpress. Usage Cited in: Aging (Albany NY). 2018 Nov 28;10(11):3353-3370. [Abstract]
- KLE cells are stained by 25 μg/mL PI in fluorescence-activated cell sorting buffer, incubated cells for 30 min in dark environment at room temperature. Cell apoptosis is determined by Annexin V/PI double staining and percentages of cells in each phase are calculated.
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Propidium Iodide purchased from MedChemExpress. Usage Cited in: Chinese Pharmacological Bulletin. 2018 May; 34(5): 620-626.
- PC12 cells are treated with 1.5 mM Methylglyoxal (MG) for 24 h before pretreatment with Butein (2.5, 5 μM) for 1 h. Propidium iodide (PI) and Hoechst 33342 are used to determine cell apoptosis.
Description |
Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis. |
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In Vitro |
"General Protocol MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Masse moléculaire |
668.39 |
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Formule |
C27H34I2N4 |
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CAS No. |
25535-16-4 |
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Appearance |
Solid |
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Color |
Pink to red |
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Emission (Em) |
615 |
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Excitation (Ex) |
535 |
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SMILES |
C[N+](CCC[N+]1=C(C2=CC=CC=C2)C3=CC(N)=CC=C3C4=C1C=C(N)C=C4)(CC)CC.[I-].[I-] |
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Livraison | Room temperature in continental US; may vary elsewhere. |
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Stockage |
4°C, sealed storage, away from moisture and light *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light) |
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Solvant et solubilité |
In Vitro:
DMSO : 100 mg/mL (149.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : 3.57 mg/mL (5.34 mM; ultrasonic and warming and heat to 60°C) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Pureté et documentation | |||||||||||||
Dyeing Example |
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Références |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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H2O / DMSO | 1 mM | 1.4961 mL | 7.4807 mL | 14.9613 mL | 37.4033 mL |
5 mM | 0.2992 mL | 1.4961 mL | 2.9923 mL | 7.4807 mL | |
DMSO | 10 mM | 0.1496 mL | 0.7481 mL | 1.4961 mL | 3.7403 mL |
15 mM | 0.0997 mL | 0.4987 mL | 0.9974 mL | 2.4936 mL | |
20 mM | 0.0748 mL | 0.3740 mL | 0.7481 mL | 1.8702 mL | |
25 mM | 0.0598 mL | 0.2992 mL | 0.5985 mL | 1.4961 mL | |
30 mM | 0.0499 mL | 0.2494 mL | 0.4987 mL | 1.2468 mL | |
40 mM | 0.0374 mL | 0.1870 mL | 0.3740 mL | 0.9351 mL | |
50 mM | 0.0299 mL | 0.1496 mL | 0.2992 mL | 0.7481 mL | |
60 mM | 0.0249 mL | 0.1247 mL | 0.2494 mL | 0.6234 mL | |
80 mM | 0.0187 mL | 0.0935 mL | 0.1870 mL | 0.4675 mL | |
100 mM | 0.0150 mL | 0.0748 mL | 0.1496 mL | 0.3740 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Propidium Iodide Related Classifications
- Others
- Others Cell Cycle/DNA Damage
- Fluorescent Dye DNA/RNA Synthesis