Recombinant Rat TNF-α (P. pastoris)

Biological Activity

Tumor necrosis factor-α (TNF-α) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. In addition to inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, rheumatoid arthritis, and graft-versus-host disease. Tnf-1a is a potent lymphofactor that has cytotoxic effects on a variety of tumor cells and some other target cells. 

Accession #: P06804 

Apparent molecular weight: about 17.4kDa on SDS-PAGE under reduced condition 

Endotoxin: < 0.2 EU/μg 

Lyophilized after extensive dialysis against PBS.

Product Citations

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  Biomater Res. 2024 Mar 4;28:0006.

  CD62E- and ROS-Responsive ETS Improves Cartilage Repair by Inhibiting Endothelial Cell Activation through OPA1-Mediated Mitochondrial Homeostasis
  Recombinant Rat TNF-α (P. pastoris) purchased from AbMole

Chemical Information

Solubility (25°C)	Reconstitute the lyophilized powder in ddH2O up to 100 μg/ml.
Storage	Stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C.

References

[1] Mingde Ji, et al. PLoS One. C5a Induces the Synthesis of IL-6 and TNF-α in Rat Glomerular Mesangial Cells through MAPK Signaling Pathways

[2] Xu-Hong Wei, et al. Exp Neurol. Peri-sciatic administration of recombinant rat TNF-alpha induces mechanical allodynia via upregulation of TNF-alpha in dorsal root ganglia and in spinal dorsal horn: the role of NF-kappa B pathway

[3] G S Rees, et al. Eur Cytokine Netw. Rat tumour necrosis factor-alpha: expression in recombinant Pichia pastoris, purification, characterization and development of a novel ELISA

[4] A K Olejnik, et al. Immunol Lett. Tumor necrosis factor alpha (TNF-alpha) modulates rat mast cell reactivity

[5] P M Hopkins. Intensive Care Med. Human recombinant TNF alpha affects rat diaphragm muscle in vitro