HY-B0176A-10mg | Sertraline (hydrochloride) [79559-97-0] Clinisciences

Description

Sertraline hydrochloride is an antidepressant of the selective serotonin reuptake inhibitor (SSRI) class. Sertraline hydrochloride is researched for a number of diseases, such as major depressive disorder and obsessive^[1]^[2].

In Vitro

Sertraline (1 μM, 12 hours) inhibits high mobility group box 1 (HMGB1)- or tumor necrosis factor-α (TNF-α)-induced microglial activation and inflammatory mediator production in microglia^[3].
Sertraline (1 μM) inhibits the HMGB1/toll-like receptor 4 (TLR4)- and TNF-α/TNF receptor 1 (TNFR1)-mediated nuclear factor-kappa B (NF-κB) activation in HMGB1- or TNF-α-stimulated microglia^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Sertraline (10 mg/kg) is shown to produce the optimal anti-depression effectsin chronic unpredictable mild stress (CUMS)-exposed mice^[3].
Sertraline (10 mg/kg; a single i.p.) significantly reduces the immobility time during forced swim test (FST) in all female animals^[4].
Sertraline (10 mg/kg; a single i.p.) does not alter spontaneous locomotor activity in all three genotypes regardless of sex difference^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Aromatase-knockout (Ar^−/−) mice^[4]
Dosage:	10 mg/kg
Administration:	A single i.p. administration
Result:	Reduced the immobility time during FST in all genotypes, except male Ar^+/−mice.

Essai clinique

Masse moléculaire

342.69

Formule

C₁₇H₁₈Cl₃N

CAS No.

79559-97-0

Appearance

Solid

Color

White to off-white

SMILES

CN[C@H]1CC[C@@H](C2=CC=C(Cl)C(Cl)=C2)C3=C1C=CC=C3.Cl

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

Solvant et solubilité

In Vitro:

DMSO : 50 mg/mL (145.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 2.86 mg/mL (8.35 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9181 mL	14.5904 mL	29.1809 mL
5 mM	0.5836 mL	2.9181 mL	5.8362 mL
10 mM	0.2918 mL	1.4590 mL	2.9181 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculateur de molarité
Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureté et documentation

Purity: 99.96%

Select Batch:

Fiche technique (278 KB) SDS (393 KB)

COA (250 KB) LCMS (90 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Murdoch D, et al. Sertraline. A review of its pharmacodynamic and pharmacokinetic properties, and therapeutic potential in depression and obsessive-compulsive disorder. Drugs. 1992 Oct;44(4):604-24. [Content Brief]

[2]. Koe BK. Preclinical pharmacology of sertraline: a potent and specific inhibitor of serotonin reuptake. J Clin Psychiatry. 1990 Dec;51 Suppl B:13-7. [Content Brief]

[3]. Xiang X, et, al. Anti-depressive Effect of Arctiin by Attenuating Neuroinflammation via HMGB1/TLR4- And TNF-α/TNFR1-mediated NF-κB Activation. ACS Chem Neurosci. 2020 Jul 1. [Content Brief]

[4]. Lei M, et, al. Sex Differences in Antidepressant Effect of Sertraline in Transgenic Mouse Models. Front Cell Neurosci. 2019 Feb 1; 13:24. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.9181 mL	14.5904 mL	29.1809 mL	72.9522 mL
H₂O / DMSO	5 mM	0.5836 mL	2.9181 mL	5.8362 mL	14.5904 mL
DMSO	10 mM	0.2918 mL	1.4590 mL	2.9181 mL	7.2952 mL
	15 mM	0.1945 mL	0.9727 mL	1.9454 mL	4.8635 mL
	20 mM	0.1459 mL	0.7295 mL	1.4590 mL	3.6476 mL
	25 mM	0.1167 mL	0.5836 mL	1.1672 mL	2.9181 mL
	30 mM	0.0973 mL	0.4863 mL	0.9727 mL	2.4317 mL
	40 mM	0.0730 mL	0.3648 mL	0.7295 mL	1.8238 mL
	50 mM	0.0584 mL	0.2918 mL	0.5836 mL	1.4590 mL
	60 mM	0.0486 mL	0.2432 mL	0.4863 mL	1.2159 mL
	80 mM	0.0365 mL	0.1824 mL	0.3648 mL	0.9119 mL
	100 mM	0.0292 mL	0.1459 mL	0.2918 mL	0.7295 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Sertraline (hydrochloride) [79559-97-0]

Cat# HY-B0176A-10mg

Contatta il distributore locale :

Marca : MedChemExpress

Contatta il distributore locale :

Other Forms of Sertraline hydrochloride:

Calculateur de molarité

Calculateur de dilution

Complete Stock Solution Preparation Table

Sertraline hydrochloride Related Classifications

Keywords:

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Sertraline (hydrochloride) [79559-97-0]

Cat# HY-B0176A-10mg

Contatta il distributore locale :

Marca : MedChemExpress

Contatta il distributore locale :

Other Forms of Sertraline hydrochloride:

Sertraline hydrochloride Related Antibodies

Calculateur de molarité

Calculateur de dilution

Complete Stock Solution Preparation Table

Sertraline hydrochloride Related Classifications

Keywords:

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