This kit contains reagents for performing tyramide signal amplification (TSA) using goat anti-mouse HRP secondary antibody and fluorescent CF® Dyes or biotin to increase immunofluorescence signal.

Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ.  The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to conventional avidin-biotin detection. Multiple rounds of tyramide signal amplification can be performed for multiplex detection; to learn more, see our Tech Tip: Multicolor Fluorescence Imaging using Tyramide Amplification Kits. Learn more about tyramide signal amplification.

The HRP goat anti-mouse antibody provided in the kits has been adsorbed against bovine, horse, human, rabbit, and swine serum to minimize cross-reactivity; the antibody is not cross-adsorbed against rat. The kits include your choice of 6 CF® dye tyramides or biotin tyramide. CF® dyes are Biotium’s line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes. Learn more about CF® dyes.

Also see our Tyramide Amplification Kits with HRP Goat Anti-Rabbit and Tyramide Amplification Kits with HRP Streptavidin.

Each kit contains reagents for staining 50-150 slides; the actual number of samples that can be stained will depend on staining volumes used. Tyramide Amplification Buffer, CF® Dye Tyramides, and other tyramide compounds are also available separately. See our full selection of tyramide products.

Tyramide Signal Amplification Kits

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