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> Vitamin B6 ELISA Kit

Vitamin B6 ELISA Kit

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Cat# OKEH02570

Size : 96Wells

Marca : Aviva Systems Biology

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Vitamin B6 ELISA Kit (OKEH02570)

ELISA Standard Curve
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02570
Product Info
Predicted Species ReactivityAll Species
ApplicationELISA-Comp
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Vitamin B6 ELISA Kit (OKEH02570) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Vitamin B6 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Vitamin B6 and incubated. The Vitamin B6 found in the sample or standards competes with the biotinylated Vitamin B6 for limited binding sites on the immobilized anti-Vitamin B6 antibody. Excess unbound biotinylated Vitamin B6 and sample or standard Vitamin B6 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Vitamin B6 captured in the well and inversely proportional to the amount of Vitamin B6 which was contained in the sample or standard.
ELISA Kit Range3.12-200nmol/L
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Vitamin B6 Microplate96 Wells (12 x 8 Well strips)
Vitamin B6 Lyophilized Standard2 x 200 nmol
Sample Diluent1 x 20 mL
100X Vitamin B6-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: VB6
Target Name: Vitamin B6
::Pubchem: 1054
::Chemical formula: C8H11NO3
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity1.26nmol/L
SpecificityNatural and recombinant General Vitamin B6
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsVB6, Vitamin B6, VB6
Protein NameVitamin B6
Description of TargetVitamin B6 is a water-soluble vitamin and is part of the vitamin B complex group. Several forms of the vitamin are known, but pyridoxal phosphate (PLP) is the active form and is a cofactor in many reactions of amino acid metabolism, including transamination, deamination, and decarboxylation. PLP also is necessary for the enzymatic reaction governing the release of glucose from glycogen.