Gluconeogenesis








Gluconeogenesis takes place in the liver, it consists of eleven enzyme-catalyzed reactions. Many of the reactions are the reversible steps found in glycolysis. Three reactions are replaced with more kinetically favorable reactions. Thus the glycolytic enzymes pyruvate kinase, phosphofructokinase, and hexokinase/glucokinase are replaced with PEP carboxykinase, fructose-1,6-bisphosphatase and glucose-6-phosphatase.

Pyruvate

Please find assay kits for pyruvic acid.

Pyruvate carboxylase

Gluconeogenesis begins in the mitochondria with the formation of oxaloacetate by the carboxylation of pyruvate via the pyruvate carboxylase. Please find for Pyruvate carboxylase: antibodies, cDNA clones, peptides, ELISA kits, blocking peptides, assay kits.

Oxaloacetate

Please find assay kits for oxaloacetate.

PEP carboxykinase

Oxaloacetate is decarboxylated and then phosphorylated to form phosphoenolpyruvate using the enzyme phosphoenolpyruvate carboxykinase (PEPCK). Please find for PEPCK: cDNA clones, ELISA kits, antibodies, peptides, assay kits.

Phosphoenolpyruvate

Please find assay kits for Phosphoenolpyruvate.

Enolase

Enolase allows transformation of Phosphoenolpyruvate into 2-phosphoglycerate. Please find for enolase: antibodies, peptides, cDNA clones, blocking peptides, RNAi, assay kits.

2-phosphoglycerate

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Phosphoglycerate mutase

Phosphoglycerate mutase allows the conversion of 2-phosphoglycerate into 3-phosphoglycerate. Please find for Phosphoglycerate mutase: cDNA clones, peptides, antibodies, ELISA kits, assay kits.

3-phosphoglycerate

Phosphoglycerate kinase

Phosphoglycerate kinase allows conversion of 3-phosphoglycerate into 1,2-bisphosphoglycerate. Please find for phosphoglycerate kinase: antibodies, cDNA clones, peptides, ELISA kits.

1,2-bisphosphoglycerate

Glyceraldehyde phosphate dehydrogenase

Please find for Glyceraldehyde phosphate dehydrogenase: cDNA clones, antibodies, peptides, ELISA kits.

Glyceraldehyde 3-phosphate

Triosephosphate isomerase

Please find for triosephosphate isomerase: antibodies, cDNA clones, peptides, ELISA kits,  assay kits.

Dihydroxyacetone phosphate

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Aldolase

Please find for Fructose bisphosphate aldolase: ELISA kits, peptides, antibodies, cDNA clones.

Fructose-1,6-bisphosphate

Fructose-1,6-bisphosphate lies within the glycolysis metabolic pathway.

Fructose-1,6-bisphosphatase

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Fructose-6-phosphate

Please find our assay kits for Fructose-6-phosphate.

Glucose-6-phosphate isomerase

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Glucose-6-phosphate

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Glucose-6-phosphatase

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Glucose

Please find for Glucose: assay kits.