
Tampons de dilution
Tampons de dilution d'anticorps, de standards de calibration et d'échantillons pour les immunoessais
Les tampons de dilution sont conçus pour éviter les interférences dans les immunoessais qui pourraient donner des résultats faussement positifs ou négatifs.
Si vous avez un important bruit de fond ou des faux-positifs, ceci est peut-être dû à des phénomènes de réactivité croisée ou à des effets de la matrice. Il est alors recommandé d'utiliser un tampon de dilution adéquate pour palier à ces problèmes.
Les anticorps, les standards pour la calibration ainsi que les échantillons comme le plasma, le sérum, la salive, les selles ou de la nourriture peuvent être dilués directement avec un tampon de dilution.
Nous proposons une gamme de tampons de dilution pour différentes applications avec ou sans tween. En effet, il est recommandé de ne pas utiliser de tween pour l'immunohistochimie.
Il est important de choisir le bon tampon de dilution afin d’optimiser la liaison entre les anticorps et leurs cibles.
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- AP-staining. Click here to download PDF datasheet. Target Protein Principal Names: CD74 1
- CD74 antigen 1
- CD74 antigen (invariant polypeptide of major histocompatibility complex 1
- CD74 molecule 1
- DHLAG 1
- EBP12552 - Immunizing Peptide 1
- Goat Anti-CD74 Antibody Code Name Applications Availability Size Tested species Price Grade EB12551 Goat Anti-CD74 (aa159-171) Antibody Pep-ELISA 1
- HLA-DR-gamma 1
- HLADG 1
- IHC 100µg specific antibody in 200µl Human $324.00 Ordering Information for United States of America (change) Order direct from Everest $49.00: Overnight shipping (not applicable to SB-shifix) to United States of America Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC. Order offline OR Order from our local distributor ARP American Research Products 1
- Ia-GAMMA 1
- Ia-associated invariant chain 1
- Inc View contact details Product details Reviews Protocols and MSDS EB12551 (0.01µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence. EB12551 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer Ph 6 1
- MHC HLA-DR gamma chain 1
- NP_004346.1 Human GeneID(s): 972 Important Comments: This antibody is expected to recognize isoform a (NP_001020330.1) and isoform b (NP_004346.1). Immunogen Peptide with sequence C-KGSFPENLRHLKN 1
- NP_004346.1. Please note the peptide is available for sale. Purification and Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline 1
- Search Type in product code or name: Goat Anti-CD74 (aa159-171) Antibody (EB12551) Related Products: EBP12551 - Immunizing Peptide 1
- WB 1
- class II antigen-associated) 1
- class II invariant chain 1
- from the internal region of the protein sequence according to NP_001020330.1 1
- gamma chain of class II antigens Official Symbol: CD74 Accession Number(s): NP_001020330.1 1
- major histocompatibility complex 1
- pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. Applications Tested Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 33kDa band observed in Human Tonsil lysates (calculated MW of 33.5kDa according to NP_001020330.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml. Species Reactivity Tested: Human Expected from sequence similarity: Human 1
Référence
Description
Cond.
Prix HT
NB-27-00082
1ml (4µM)
NB-27-00083
0.5ml (4µM)
LDR-50
50ml
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