γ-Glutamyl Transferase (GGT/γ-GT) Activity Assay Kit

Referentie E-BC-K126-M-96T

Formaat : 96T

Merk : Elabscience

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Detection Principle γ-GT catalyzes the transfer of gamma glutamyl group from glutamyl p-nitroaniline to N-glycyl glycine to produce p-nitroaniline, which has characteristic absorption peak at 405 nm. The activity of γ-GT can be calculated according to the changing rate of absorbance value.
Synonyms GGT;γ-GT
Sample Type Serum,Plasma,Animal tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (405 nm)
Research Area Liver And Renal Function
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.88 U/L
Detection Range 0.88-399.4 U/L
Precision inter-assay CV: 6.2% | intra-assay CV: 4.2%
Assay Time 20 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Mouse serum 1
Human serum 1
Rat serum 1
Dog serum 1
Human plasma 1
Horse serum 1
Porcine serum 1
Human hydrothorax 1
10% Mouse liver tissue homogenate 1
10% Mouse heart tissue homogenate 1
10% Rat spleen tissue homogenate 1
10% Rat lung tissue homogenate 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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E-BC-K168-M-96T
 96T