The 2.5% Trypsin-EDTA Solution 10X is a concentrated enzymatic preparation commonly used in cell culture research to dissociate adherent cells from the substrate or each other, facilitating their harvest and passage. Trypsin, a serine protease derived from porcine pancreas, cleaves peptide bonds in proteins, targeting primarily those on the carboxyl side of lysine and arginine residues. This action helps to break down the protein structures that hold cells together or attach them to the culture dish surface. EDTA, or ethylenediaminetetraacetic acid, is included in this solution to enhance the effectiveness of trypsin. EDTA acts as a chelating agent, binding divalent cations such as calcium and magnesium that are critical for cell adhesion. By removing these ions, EDTA reduces the strength of cell-to-cell and cell-to-substrate adhesion, thus synergistically working with trypsin to facilitate cell detachment without excessive damage. In scientific research, this trypsin-EDTA solution is crucial for cell culture applications, particularly when researchers need to maintain cell viability and functionality post-harvest. The enzymatic action allows for the gentle separation of cells, preserving important surface receptors and cell integrity, which is essential for downstream applications like flow cytometry, cell sorting, and tissue engineering studies. The 10X concentration provides a convenient stock solution that can be diluted to the desired strength based on specific experimental needs, offering flexibility and efficiency in experimental workflows.

References:

1. [Long-term in vitro culture of the fibroblasts from the deep partial thickness burn wound in burn patients].  |  Li, YL., et al. 2003. Zhonghua Shao Shang Za Zhi. 19: 35-7. PMID: 12678974
2. Increased proteolysis of collagen in an in vitro tensile overload tendon model.  |  Willett, TL., et al. 2007. Ann Biomed Eng. 35: 1961-72. PMID: 17763961
3. Different methods of detaching adherent cells significantly affect the detection of TRAIL receptors.  |  Zhang, B., et al. 2012. Tumori. 98: 800-3. PMID: 23389369
4. Biophysical characterization of low-frequency ultrasound interaction with dental pulp stem cells.  |  Ghorayeb, SR., et al. 2013. J Ther Ultrasound. 1: 12. PMID: 25516801
5. Atomic force microscopy in the production of a biovital skin graft based on human acellular dermal matrix produced in-house and in vitro cultured human fibroblasts.  |  Łabuś, W., et al. 2018. J Biomed Mater Res B Appl Biomater. 106: 726-733. PMID: 28323389
6. A novel autophagy modulator 6-Bio ameliorates SNCA/ alpha -synuclein toxicity.  |  Suresh, SN., et al. 2017. Autophagy. 13: 1221-1234. PMID: 28350199
7. Development of a new method for the preparation of an acellular allodermis, quality control and cytotoxicity testing.  |  Dragúová, J., et al. 2017. Cell Tissue Bank. 18: 153-166. PMID: 28405854
8. Specific capture, recovery and culture of cancer cells using oriented antibody-modified polystyrene chips coated with agarose film.  |  Jeong, J., et al. 2018. Colloids Surf B Biointerfaces. 162: 306-315. PMID: 29220830
9. A simple trypsin resistance assay for muscle and other cell fusion.  |  Denning, G. and Fulton, AB. 1986. J Histochem Cytochem. 34: 959-62. PMID: 3086428
10. Collagen Fibrils Mechanically Contribute to Tissue Contraction in an In Vitro Wound Healing Scenario.  |  Brauer, E., et al. 2019. Adv Sci (Weinh). 6: 1801780. PMID: 31065517
11. Estimation and in-situ detection of thorium in human liver cell culture by arsenazo-III based colorimetric assay.  |  Yadav, R., et al. 2020. Biometals. 33: 75-85. PMID: 31897857
12. Establishing Sustainable Cell Lines of a Coral, Acropora tenuis.  |  Kawamura, K., et al. 2021. Mar Biotechnol (NY). 23: 373-388. PMID: 33899125
13. Morphologic changes in rat urothelial cells during carcinogenesis: I. Histologic and cytologic changes.  |  King, EB., et al. 1984. Cytometry. 5: 447-53. PMID: 6489059
14. Primary culture of bovine gall bladder epithelial cells.  |  Plevris, JN., et al. 1993. Gut. 34: 1612-5. PMID: 8244152