Component | AS111-01/11 | AS111-02/12 | AS111-03/13 | AS111-14 |
2×EasyTaq® PCR SuperMix (-dye)/(+dye) | 1 ml | 5×1 ml | 15×1 ml | 6×80 ml |
Nuclease-free Water | 1 ml | 5 ml | 3×5 ml | 96×5 ml |
2×EasyTaq® PCR SuperMix (-dye)
Referentie AS111-03
Formaat : 15×1ml
Merk : TransGen Biotech
2×EasyTaq® PCR SuperMix (+dye )
Catalog Number: AS111-11
Price:Please inquire first
Product Details
EasyTaq® PCR SuperMix is a ready-to-use mixture of EasyTaq® DNA Polymerase, dNTPs, and optimized reaction buffer at a concentration of 2×. For DNA amplification, simply add the template, primers, and water to allow the SuperMix solution to react at a concentration of 1×. For 2×EasyTaq® PCR SuperMix (-dye), the PCR products which contain dA overlangs at the 3' end can be directly cloned to pEASY®-T vectors. For 2×EasyTaq® PCR SuperMix (+dye), the PCR products can be analyzed by electrophoresis directly, and need to be purified to remove dye when applied in cloning. Please note, PCR products using this SuperMix are not suitable for polyacrylamide gel electrophoriesis (PAGE).
Highlights
• Reduced reaction setup time
• Minimized contamination caused by multi-step operation.
• High amplification efficiency and stability.
• Amplification of genomic DNA fragment up to 4 kb.
Applications
Routine PCR
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
1 Liu W, Yao Q, Su X, et al. Molecular insights into Spindlin1-HBx interplay and its impact on HBV transcription from cccDNA minichromosome[J]. Nature Communications, 2023.(IF 16.60)
2 Li C, Chen X, Wen R, et al. Immunocapture magnetic beads enhanced the LAMP-CRISPR/Cas12a method for the sensitive, specific, and visual detection of Campylobacter jejuni[J]. Biosensors, 2022.(IF 5.50)
3 Wang X, Liu C, Zhang S, et al. N6-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles[J]. Developmental cell, 2021.(IF 10.09)
4 Li D, Liu Y, Yi P, et al. RNA editing restricts hyperactive ciliary kinases[J]. Science, 2021.(IF 63.71)
5 Han D, Liu J, Chen C, et al. Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells[J]. Nature, 2019.(IF 64.8)