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> 6-Benzylaminopurine [1214-39-7]

6-Benzylaminopurine [1214-39-7]

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6-Benzylaminopurine (Benzyladenine) is the first cytokinin that causes plant growth and development by stimulating cell division and inhibiting respiratory kinases, thereby prolonging the preservation of green vegetables.

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6-Benzylaminopurine Chemical Structure

6-Benzylaminopurine Chemical Structure

CAS No. : 1214-39-7

This product is a controlled substance and not for sale in your territory.

Based on 3 publication(s) in Google Scholar

Other Forms of 6-Benzylaminopurine:

  • 6-Benzylaminopurine-d5 In-stock
  • 6-Benzylaminopurine, 98% hydrochloride Obtenir un devis
Description

6-Benzylaminopurine (Benzyladenine) is the first cytokinin that causes plant growth and development by stimulating cell division and inhibiting respiratory kinases, thereby prolonging the preservation of green vegetables[1].

In Vivo

6-Benzylaminopurine (100 μM, when the cotyledons were fully expanded and the frst true leaf was just emerged, each seedling was sprayed every other day until it reached 5-true-leaf stage. Then, each plant was sprayed twice per week until fruit set) results in an increase in wart density by 107.8% in Cshec2 knockout fruits of cucumber[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Masse moléculaire

225.25

Formule

C12H11N5
CAS No.

1214-39-7
Appearance

Solid

Color

White to off-white

SMILES

C12=NC=NC(NCC3=CC=CC=C3)=C1NC=N2
Structure Classification
  • Others
Initial Source
  • Animals

Apis cerana
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvant et solubilité
In Vitro: 

DMSO : 50 mg/mL (221.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4395 mL 22.1976 mL 44.3951 mL
5 mM 0.8879 mL 4.4395 mL 8.8790 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (11.10 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (11.10 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation

Purity: 99.97%

COA (250 KB) HNMR (131 KB) RP-HPLC (258 KB)

Instruction de manipulation (2659 KB)
Références

  • [1]. Wang Z, et al. HECATE2 acts with GLABROUS3 and Tu to boost cytokinin biosynthesis and regulate cucumber fruit wart formation. Plant Physiol. 2021 Nov 3;187(3):1619-1635.  [Content Brief]

  • [1]. Wang Z, et al. HECATE2 acts with GLABROUS3 and Tu to boost cytokinin biosynthesis and regulate cucumber fruit wart formation. Plant Physiol. 2021 Nov 3;187(3):1619-1635.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.4395 mL 22.1976 mL 44.3951 mL 110.9878 mL
5 mM 0.8879 mL 4.4395 mL 8.8790 mL 22.1976 mL
10 mM 0.4440 mL 2.2198 mL 4.4395 mL 11.0988 mL
15 mM 0.2960 mL 1.4798 mL 2.9597 mL 7.3992 mL
20 mM 0.2220 mL 1.1099 mL 2.2198 mL 5.5494 mL
25 mM 0.1776 mL 0.8879 mL 1.7758 mL 4.4395 mL
30 mM 0.1480 mL 0.7399 mL 1.4798 mL 3.6996 mL
40 mM 0.1110 mL 0.5549 mL 1.1099 mL 2.7747 mL
50 mM 0.0888 mL 0.4440 mL 0.8879 mL 2.2198 mL
60 mM 0.0740 mL 0.3700 mL 0.7399 mL 1.8498 mL
80 mM 0.0555 mL 0.2775 mL 0.5549 mL 1.3873 mL
100 mM 0.0444 mL 0.2220 mL 0.4440 mL 1.1099 mL
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6-Benzylaminopurine Related Classifications

  • Others
  • Metabolic Enzyme/Protease
  • Endogenous Metabolite
Help & FAQs

Keywords:

6-Benzylaminopurine1214-39-7Benzyladenine 6-BAP N6-BenzyladenineEndogenous Metabolitecytokininplant growthcell divisionInhibitorinhibitorinhibit

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