acetylated α Tubulin (6-11B-1) FITC

Referentie sc-23950 FITC

Formaat : 200µg/ml

Merk : Santa Cruz Biotechnology

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Anticorps acetylated alpha Tubulin (6-11B-1): sc-23950

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Fiches techniques
  • L'anticorps acetylated alpha Tubulin 6-11B-1 est un monoclonal IgG2b κ L'Anticorps acetylated alpha Tubulin, cité dans 325 publications, fourni en 200 µg/ml
  • raised against the outer arms of Strongylocentrotus purpuratus (Sea Urchin) sperm axonemes
  • L'Anticorps acetylated alpha Tubulin (6-11B-1) est recommandé pour la détection de acetylated α Tubulin d'origine mammalian species, zebrafish, Drosophila et Xenopus par WB, IP, IF et IHC(P); non cross-reactif avec non-acetylated α Tubulin or other lysine acetylation sites
  • Anti-L'Anticorps acetylated alpha Tubulin (6-11B-1) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
  • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
  • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
  • m-IgG Fc BP-HRP et 2b BP-HRP">m-IgG2b BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps acetylated alpha Tubulin (6-11B-1) pour des applications de WB and IHC(P). Ces réactifs sont maintenant proposés en kit avec l'anticorps acetylated alpha Tubulin (6-11B-1) ().

ACCÈS RAPIDE AUX LIENS

, α 3 Tubulin, α 4 Tubulin, α Tubulin, β Tubulin, β 2 Tubulin, β 3 Tubulin, δ Tubulin, ε Tubulin et γ Tubulin

L'anticorps α-tubuline acétylée (6-11B-1) est un anticorps monoclonal de souris IgG2b κ alpha-tubuline acétylée qui détecte la protéine alpha-tubuline acétylée des espèces de mammifères, du poisson zèbre, de la drosophile et de Xenopus par WB, IP, IF et IHC(P). L'anticorps α-tubuline acétylée (6-11B-1) est disponible sous forme d'anticorps anti-tubuline alpha non conjugué, ainsi que sous plusieurs formes conjuguées d'anticorps anti-tubuline alpha acétylée, y compris agarose, HRP, PE, FITC et plusieurs conjugués Alexa Fluor®. La tubuline est un composant majeur du cytosquelette qui possède cinq formes distinctes, désignées α, β, γ, δ et ε Tubuline. α et β Tubuline forment des hétérodimères qui se multimérisent pour former un filament de microtubules. Plusieurs isoformes de β-tubuline (β1, β2, β3, β4, β5, β6 et β8) ont été caractérisées et sont exprimées dans les tissus des mammifères. Les protéines β1 et β4 sont présentes dans tout le cytosol, la protéine β2 est présente dans les noyaux et le nucléoplasme, et la protéine β3 est une protéine du cytosquelette spécifique des neurones. La γ tubuline forme le gammasome, qui est nécessaire à la nucléation des filaments de microtubules au niveau du centrosome. La tubuline δ et la tubuline ε sont toutes deux associées au centrosome. La δ Tubuline est un homologue de la δ Tubuline Uni3 de Chlamydomonas et se trouve en association avec les centrioles, tandis que la ε Tubuline se localise dans le matériel péricentriolaire. La ε tubuline présente un schéma de localisation spécifique au cycle cellulaire, s'associant d'abord uniquement avec le plus ancien des centrosomes d'une paire nouvellement dupliquée et s'associant ensuite avec les deux centrosomes.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Alexa Fluor® est une marque déposée de Molecular Probes Inc., OR., USA

LI-COR® et Odyssey® sont marques déposées de LI-COR Biosciences

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps acetylated alpha Tubulin (6-11B-1)

sc-23950
200 µg/ml
.00

acetylated alpha Tubulin (6-11B-1): m-IgG Fc BP-HRP Kit

sc-525509
200 µg Ab; 10 µg BP
.00

acetylated alpha Tubulin (6-11B-1): m-IgG2b BP-HRP Kit

sc-549568
200 µg Ab; 10 µg BP
.00

Anticorps acetylated alpha Tubulin (6-11B-1) AC

sc-23950 AC
500 µg/ml, 25% agarose
.00

Anticorps acetylated alpha Tubulin (6-11B-1) HRP

sc-23950 HRP
200 µg/ml
.00

Anticorps acetylated alpha Tubulin (6-11B-1) FITC

sc-23950 FITC
200 µg/ml
.00

Anticorps acetylated alpha Tubulin (6-11B-1) PE

sc-23950 PE
200 µg/ml
.00

Anticorps acetylated alpha Tubulin (6-11B-1) Alexa Fluor® 488

sc-23950 AF488
200 µg/ml
.00

Anticorps acetylated alpha Tubulin (6-11B-1) Alexa Fluor® 546

sc-23950 AF546
200 µg/ml
.00

Anticorps acetylated alpha Tubulin (6-11B-1) Alexa Fluor® 594

sc-23950 AF594
200 µg/ml
.00

Anticorps acetylated alpha Tubulin (6-11B-1) Alexa Fluor® 647

sc-23950 AF647
200 µg/ml
.00

Anticorps acetylated alpha Tubulin (6-11B-1) Alexa Fluor® 680

sc-23950 AF680
200 µg/ml
.00

Anticorps acetylated alpha Tubulin (6-11B-1) Alexa Fluor® 790

sc-23950 AF790
200 µg/ml
.00

1. What dilution works well for acetylated alpha Tubulin Antibody (6-11B-1) Alexa Fluor® 488 in PBMCs? 2. What isotype control is suitable for this conjugated antibody?

Posée par: Gautam
Thank you for contacting Santa Cruz Biotechnology . We would need to know what application the antibody is used for to better assist you. For help please contact scbt@scbt.com. Here are the controls we offer :https://www.scbt.com/browse/Control-IgGs-and-IgG-Conjugates/_/N-1j3ajah
Répondue par: BlakeJ
Date de publication: 2024-05-14

We have lot # HQ422 of prodfuct sc-23950. I am getting a very week signal at 1:200 on fixed airway epithelial cells. Is this lot expired? Thanks. Shubha

Posée par: Shubha
Thank you for your question. We warranty our antibodies for 1 year from date of purchase . Lot HQ422 is not a valid lot number . Please give us a call or email us to discuss your results. scbt@scbt.com or (800)457-3801.
Répondue par: BlakeJ
Date de publication: 2023-12-01

The product specifications list amino acids 149-448, however, in previous questions it was noted that this product recognizes Ac-K40. Can you please confirm which epitope this antibody recognizes?

Posée par: Laura
Thank you for your question. The epitope range is 149-448.
Répondue par: BlakeJ
Date de publication: 2023-11-14

Hi, interested in buying an anti-acetyl-alpha Tubulin antibody to test the effect of HDAC8 inhibitors by WB. Wondering if I could purchase or get an small aliquot to test it before placing the order of a full product

Posée par: CarlosPR
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Répondue par: Dallas Tech Service
Date de publication: 2021-04-07

We have purchased and used acetylated α Tubulin Antibody (6-11B-1): sc-23950 antibody. It is working quite well. However we need to know the which amino acid residue the antibody is reacting with? Kindly tell me particular epitope to which ab binds.

Posée par: Nitish
Thank you for your question. This mouse monoclonal antibody recognizes endogenous levels of α-tubulin protein only when acetylated at Lys40 (Lysine 40).
Répondue par: Technical Support
Date de publication: 2024-11-07

Hi, I would like to know which light chain is in this antibody (e.g, kappa I, II or V). I would like to undertsand if I can purify the antibody with protein L resin. I would like to find out the affinity of the antibody with protein L. Thank you!

Posée par: Foram
Thank you for your question. Since acetylated α Tubulin Antibody (6-11B-1): sc-23950 is a mouse IgG2 antibody it can be purified using Protein A or Protein A/G. For further information please contact our technical service directly.
Répondue par: Technical Support Europe
Date de publication: 2019-02-04

Hi, I am interested in co-staining all microtubules (a-tubulin ab) and acetylated ones (acetylated a-tubulin). The issue is that both are mouse. Would a mouse secondary detect a conjugated acetylated a-tub antibody?

Posée par: JuanManuelGomez
Thank you for your question. We would not recommend co-staining with one conjugated primary antibody, and one un-conjugated primary antibody combined with an anti-mouse secondary antibody. There would still be the possibility of cross-reactivity between the anti-mouse IgG and the conjugated mouse monoclonal antibody. Instead, we would recommend co-staining with two conjugated primary antibodies, if both are mouse IgG.
Répondue par: Technical Support
Date de publication: 2017-05-16

Dear Santa Cruz! I would like to stain the primary cilium of HK2 cells with sc-23950. I saw a cytoskeletal network on one picture and individual cilia on the other. How can I achieve the cilia staining, with no visibility of the cytoskeleton? Thank you

Posée par: mkeszthelyi
Thank you for your question. We do not have a specific protocol for using our monoclonal acetylated α Tubulin Antibody (6-11B-1): sc-23950 for detection of individual cilium exclusively. Provided below is a link to our recommended protocol for immunofluorescence and immunoperoxidase staining. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining This antibody has also been cited in the literature at least 30 times, many of which are using the antibody for IF or IHC applications. For example, PMID's: 27062996, 26079437 and 25286302. We hope that some of these publications may be useful in identifying a protocol for your research needs.
Répondue par: Technical Support
Date de publication: 2017-03-01
  • Anticorps acetylated alpha Tubulin (6-11B-1) Questions - page 2
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Rated 4 de 5 de par acetylated alpha Tubulin Antibody (6-11B-1)Good signal at the expected molecular weight of acetylated tubulin.
Date de publication: 2024-11-05
Rated 5 de 5 de par Excellent in WB!Clear signal was detected in WB of mouse brain homogenate!
Date de publication: 2024-08-26
Rated 5 de 5 de par muy buen reconocimiento de la proteínaLo probé con una linea celular de mosquito y reconoció la proteína muy bien
Date de publication: 2023-10-03
Rated 5 de 5 de par Acet. alpha-tubulinAcet. Alpha-tubulin AlexaFluor 488 antibody was used 1/100 diluted in D-PBS/Live-Dead stain (1:1000), supplemented with 60µg/ml human IgG (Sigma Aldrich) at 8◦C for 30 min. After that, cells were washed in FACS buffer (D-PBS/0.1% BSA), fixed in 4% PFA at room temperature (RT), and analyzed by flow cytometry.
Date de publication: 2023-01-16
Rated 5 de 5 de par GREAT ANTIBODIES!Really good antibody. Whole cell lysate, Primary: 1:200, Secondary: 1:4000
Date de publication: 2022-06-14
Rated 5 de 5 de par Good antibody for ac-tubulinGood antibody for ac-tubulin detection in WB.Very clean and strong signal.
Date de publication: 2020-11-17
Rated 5 de 5 de par Primary cilia - ImmunofluorescenceThe antibody works quite well at 1/1000 in fixed cells and is highly specific.
Date de publication: 2019-07-29
Rated 5 de 5 de par Primary cilia - ImmunofluorescenceThe antibody works quite well and is specific to the target.
Date de publication: 2019-05-28
  • Anticorps acetylated alpha Tubulin (6-11B-1) Avis - page 2
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Anticorps acetylated alpha Tubulin (6-11B-1) est évalué 4.8 de 5 de 45.
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