K2102-200 assays | ADP/ATP Ratio Bioluminescence Assay Kit Clinisciences

Background

The changes in ADP/ATP ratio is used to recognized the different states of the cell death and viability. Levels of ATP and ADP levels are increased in proliferating cells but decreased in apoptotic cells. The ADP/ATP Ratio Assay Kit detects ATP and ADP levels by bioluminescent for a fast screening of apoptosis, necrosis, growth arrest, and cell proliferation simultaneously in mammalian cells. This kit utilizes luciferase to catalyze the formation of light from ATP and luciferin, and the light can measured using a luminometer or Beta Counter. ADP level is measured by its conversion to ATP that is subsequently detected using the same reaction. The assay can be fully automatic for high throughput and is highly sensitive (detects 100 mammalian cells/well).

Features & Properties

Features	Simple one-step procedure; Takes only 30 minutes; Fast and convenient.
Shipping	Gel pack.
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Storage

Store at -20°C.

Overview

Kit components

Nucleotide Releasing Buffer, ATP Monitoring Enzyme, ADP Converting Enzyme, Enzyme Reconstitution Buffer

ADP/ATP Ratio Bioluminescence Assay Kit

Referentie K2102-200 assays

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