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> Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit

Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit

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Referentie E-BC-F038-96T

Formaat : 96T

Merk : Elabscience

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Telefoonnummer : +1 850 650 7790

Detection Principle ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.
Synonyms ALT
Sample Type Serum,Plasma,Animal tissue
Detection Method Fluorometric method
Detection Instrument Fluorescence microplate reader (Ex/Em=535 nm/587 nm)
Research Area Liver And Renal Function
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.01 U/L
Detection Range 0.01-0.83 U/L
Precision inter-assay CV: 9.8% | intra-assay CV: 2.3%
Assay Time 1 h 15 min
Cat.No. Product Name Sizes
E-BC-K235-M Alanine Aminotransferase (ALT/GPT) Activity Assay Kit 96T , 48T
E-BC-K236-M Aspartate Aminotransferase (AST/GOT) Activity Assay Kit 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 10-15
Dog serum 5-10
Rat serum 10-15
10% Mouse heart tissue homogenate 100-120
10% Rat spleen tissue homogenate 10-15
10% Rat liver tissue homogenate 300-500
10% Rat kidney tissue homogenate 100-120
10% Rat lung tissue homogenate 100-120

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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