Alkaline Phosphatase (ALP) BioAssay™ ECL Kit (Bovine)

The Alkaline Phosphatase (ALP) BioAssay™ ECL Kit is designed for the in vitro sensitive quantitative measurement of ALP in bovine serum, plasma, tissue homogenates, cell lysates, cell culture superanatants and other biological fluids. ||Detection Range:|41.15-30,000pg/ml||Sensitivity:|25.3pg/ml||Intra-Assay CV: |<10% ||Inter-Assay CV: |<12%||Kit Components:|*365697A: Microtiter Plate, 1x96 wells, Pre-coated with antibody; ready to use.|*365697B: Standard, 2x1vial |365697C: Standard Diluent, 1x20ml|*365697D: Detection Reagent A (green), 1x120ul|*365697E: Detection Reagent B (red), 1x120ul |365697F: Assay Diluent A, 1x12ml|365697G: Assay Diluent B, 1x12ml|365697H: Substrate A, 1x10ml|365697J: Substrate B, 1x2ml|*365697K: Positive Control, 1x1vial, xx.xpg/ml (Lyophilized)|365697L: Wash Buffer, 30X, 1x20ml ||Storage and Stability:|Store *365697A, *365697B, *365697D, *365697E and *365697K at -20°C. After reconstitution of *365697K, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.||Assay Procedure Summary:|1. Prepare all reagents, samples and standards.|2. Add 100ul standard or sample to each well,. Incubate 1 hoour at 37ºC.|3. Aspirate and add 100ul Detection Reagent A. Incubate 1 hour at 37°C.|4. Aspirate and wash 3 times.|5. Add 100ul Detection Reagent B. Incubate 30 minutes at 37°C.|6. Aspirate and wash 5 times.|7. Add 100ul Substrate Working Solution. Incubate 10 minutes at 37°C.|8. Read RLU values immediately.