alpha 1 Antitrypsin (SERPINA1) (NM_000295) Human Untagged Clone
Referentie SC119951
Formaat : 10ug
Neem contact op met een lokale distributeur :
alpha 1 Antitrypsin (SERPINA1) (NM_000295) Human Untagged Clone
| Product Data | |
| Target Symbol | alpha 1 Antitrypsin |
|---|---|
| Synonyms | A1A; A1AT; AAT; alpha1AT; nNIF; PI; PI1; PRO2275 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | Fully Sequenced ORF >NCBI ORF sequence for NM_000295, the custom clone sequence may differ by one or more nucleotides ATGCCGTCTTCTGTCTCGTGGGGCATCCTCCTGCTGGCAGGCCTGTGCTGCCTGGTCCCTGTCTCCCTGG CTGAGGATCCCCAGGGAGATGCTGCCCAGAAGACAGATACATCCCACCATGATCAGGATCACCCAACCTT CAACAAGATCACCCCCAACCTGGCTGAGTTCGCCTTCAGCCTATACCGCCAGCTGGCACACCAGTCCAAC AGCACCAATATCTTCTTCTCCCCAGTGAGCATCGCTACAGCCTTTGCAATGCTCTCCCTGGGGACCAAGG CTGACACTCACGATGAAATCCTGGAGGGCCTGAATTTCAACCTCACGGAGATTCCGGAGGCTCAGATCCA TGAAGGCTTCCAGGAACTCCTCCGTACCCTCAACCAGCCAGACAGCCAGCTCCAGCTGACCACCGGCAAT GGCCTGTTCCTCAGCGAGGGCCTGAAGCTAGTGGATAAGTTTTTGGAGGATGTTAAAAAGTTGTACCACT CAGAAGCCTTCACTGTCAACTTCGGGGACACCGAAGAGGCCAAGAAACAGATCAACGATTACGTGGAGAA GGGTACTCAAGGGAAAATTGTGGATTTGGTCAAGGAGCTTGACAGAGACACAGTTTTTGCTCTGGTGAAT TACATCTTCTTTAAAGGCAAATGGGAGAGACCCTTTGAAGTCAAGGACACCGAGGAAGAGGACTTCCACG TGGACCAGGTGACCACCGTGAAGGTGCCTATGATGAAGCGTTTAGGCATGTTTAACATCCAGCACTGTAA GAAGCTGTCCAGCTGGGTGCTGCTGATGAAATACCTGGGCAATGCCACCGCCATCTTCTTCCTGCCTGAT GAGGGGAAACTACAGCACCTGGAAAATGAACTCACCCACGATATCATCACCAAGTTCCTGGAAAATGAAG ACAGAAGGTCTGCCAGCTTACATTTACCCAAACTGTCCATTACTGGAACCTATGATCTGAAGAGCGTCCT GGGTCAACTGGGCATCACTAAGGTCTTCAGCAATGGGGCTGACCTCTCCGGGGTCACAGAGGAGGCACCC CTGAAGCTCTCCAAGGCCGTGCATAAGGCTGTGCTGACCATCGACGAGAAAGGGACTGAAGCTGCTGGGG CCATGTTTTTAGAGGCCATACCCATGTCTATCCCCCCCGAGGTCAAGTTCAACAAACCCTTTGTCTTCTT AATGATTGAACAAAATACCAAGTCTCCCCTCTTCATGGGAAAAGTGGTGAATCCCACCCAAAAATAA 5' Read Nucleotide Sequence >OriGene 5' read for NM_000295 unedited TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCAGGTTGGAGGGG CGGCAACCTCCTGCCAGCCTTCAGGCCACTCTCCTGTGCCTGCCAGAAGAGACAGAGCTT GAGGAGAGCTTGAGGAGAGCAGGAAAGGACAATGCCGTCTTCTGTCTCGTGGGGCATCCT CCTGCTGGCAGGCCTGTGCTGCCTGGTCCCTGTCTCCCTGGCTGAGGATCCCCAGGGAGA TGCTGCCCAGAAGACAGATACATCCCACCATGATCAGGATCACCCAACCTTCAACAAGAT CACCCCCAACCTGGCTGAGTTCGCCTTCAGCCTATACCGCCAGCTGGCACACCAGTCCAA CAGCACCAATATCTTCTTCTCCCCAGTGAGCATCGCTACAGCCTTTGCAATGCTCTCCCT GGGGACCAAGGCTGACACTCACGATGAAATCCTGGAGGGCCTGAATTTCAACCTCACGGA GATTCCGGAGGCTCAGATCCATGAAGGCTTCCAGGAACTCCTCCGTACCCTCAACCAGCC AGACAGCCAGCTCCAGCTGACCACCGGCAATGGCCTGTTCCTCAGCGAGGGCCTGAAGCT AGTGGATAAGTTTTTGGAGGATGTTAAAAAGTTGTACCACTCAGAAGCCTTCACTGTCAA CTTCGGGGACACCGAAGAGGCCAAGAAACAGATCAACGATTACGTGGAGAANGGTACTCA AGGGAAAATTGTGGATTTGGTCAAGGAGCTTGACAGAGACACAGTTTTTGCTCTGGTGAA TTACATCTTCTTTAAAGGCAATGGGAGAGACCCTTTGAAGTCAAGACACCGAGAAGAGGA CTTCCGTGNACCAGTGACACCGTGAAGTGCTATGAGAGCGTTTAGCATGTTAACTCCACC TGTAGAGCTGTCACTG 3' Read Nucleotide Sequence >OriGene 3' read for NM_000295 unedited CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCAGGCAGGGACCAGCTCAACCCT TCTTTAATGTCATCCAGGGAGGGGGCCAGGGATGGAGGGGAGGGGTTGAGGAGCGAGAGG CAGTTATTTTTGGGTGGGATTCACCACTTTTCCCATGAAGAGGGGAGACTTGGTATTTTG TTCAATCATTAAGAAGACAAAGGGTTTGTTGAACTTGACCTCGGGGGGGATAGACATGGG TATGGCCTCTAAAAACATGGCCCCAGCAGCTTCAGTCCCTTTCTCGTCGATGGTCAGCAC AGCCTTATGCACGGCCTTGGAGAGCTTCAGGGGTGCCTCCTCTGTGACCCCGGAGAGGTC AGCCCCATTGCTGAAGACCTTAGTGATGCCCAGTTGACCCAGGACGCTCTTCAGATCATA GGTTCCAGTAATGGACAGTTTGGGTAAATGTAAGCTGGCAGACCTTCTGTCTTCATTTTC CAGGAACTTGGTGATGATATCGCGGGTGAGTTCATTTTCCAGGTGCTGTAGTTTCCCCTC CTCATGCAAGAAGAATATGGCCGTGGCATTTGCCCAGGTATTTCATCAGCAGCACCCAGC TGGACAGCTTCTACAGGGCTGGATGTAAACATGCCTAAACGCTTCATCATAAGCACCTTC ACGGGGGCCACCTGGCCCCGTGGAAGTCCTCTTTCTCGGTGCCCCTGACTCCAAAGGGCT CTCCCTTTGCCTTTAAAGAGATGTATTTCCCACACCAAAACTGCGTTTTTGTAACCCCCT GGACCAATCCCAATTTTCCTTGAGACCCCTCTCCACTAATCGTGGAACCGCCTCTCGCCC CCTTCGGGGCCCCCAATTTACATTAAGGCTTCTCATGTCCCCTTTTTATATCCCCAAAAA CTTATCCTCTTTCTGCCCTTCTTGAATAAAGCCCTTCCCGCGGCCCTCCCCACTCCTCCC GCCTGCCGGAGGCCGAATAATCCTTCAACN |
| Restriction Sites | NotI-NotI Plasmid Map |
| ACCN | NM_000295 |
| Insert Size | 1500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000295.3, NP_000286.3 |
| RefSeq Size | 1607 bp |
| RefSeq ORF | 1257 bp |
| Locus ID | 5265 |
| UniProt ID | P01009 |
| Cytogenetics | 14q32.13 |
| Domains | SERPIN |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein |
| Protein Pathways | Complement and coagulation cascades |
| Summary | The protein encoded by this gene is a serine protease inhibitor belonging to the serpin superfamily whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. This protein is produced in the liver, the bone marrow, by lymphocytic and monocytic cells in lymphoid tissue, and by the Paneth cells of the gut. Defects in this gene are associated with chronic obstructive pulmonary disease, emphysema, and chronic liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2020] Transcript Variant: This variant (1) encodes the same protein as the other ten variants of this gene. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |