Ammonia (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. In the liver, ammonia is converted to urea through the urea cycle. Elevated levels of ammonia in the blood (hyperammonemia) have been found in liver dysfunction (cirrhosis), while hypoammonemia has been associated with defects in the urea cycle enzymes (e.g. ornithine transcarbamylase).||Simple, direct and automation-ready procedures for measuring NH3 are popular in research and drug discovery. Ammonia assay is designed to directly measure NH3 and NH4+. In this assay, NADH is converted to NAD+ in the presence of NH3, ketoglutarate and glutamate dehydrogenase. The decrease in optical density at 340nm or fluorescence intensity at λem/ λex=450/360 nm is directly proportionate to the NH3 concentration in the sample.||Key Features:|High sensitivity and wide linear range: Use 20ul sample. Linear detection range 24-1000uM ammonia.|Homogeneous and simple procedure: Simple “mix-and-measure” procedure allows reliable quantitation of NH3 within 30 minutes.||Applications:|Direct Assays: NH3 in biological samples (e.g. serum, plasma, urine, saliva, cell culture etc).||Kit Components: (96-well plate)|A1383-57A: Assay Buffer, 1x20ml |A1383-57B: Enzyme, 1x120ul|A1383-57C: Ketogutarate, 1x120ul |A1383-57D: Standard, 1x400ul |A1383-57E: NADH Reagent (Lyophilized), 1x1ml||Storage and Stability:|Store components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.