This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Testosterone(Testo) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Testosterone(Testo).
Tested applications
ELISA
Reactivity
Equine
Range
62.5-4000 pg/mL
Concentration
100 ng/mL
Sensitivity
17.7 pg/mL
Protein Length
2h
Alternative names
T ELISA Kit, Testesteron ELISA Kit, Testeronun ELI
Note
For research use only
Application notes
Standard: 4000 pg/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse Testo protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse Testo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse Testo in the samples is then determined by comparing the OD of the samples to the standard curve