Application
| WB, IHC-P, IHC-F, ICC |
|---|---|
| Primary Accession | P11087 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Collagen alpha-1(I) chain(COL1A1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 12842 |
|---|---|
| Other Names | Collagen alpha-1(I) chain, Alpha-1 type I collagen, Col1a1, Cola1 |
| Calculated MW | 138032 MW KDa |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Rat, Mouse, By Heat Immunocytochemistry , 0.5-1 µg/ml, Mouse, Human, Rat Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Human, Rat, Mouse Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix . |
| Tissue Specificity | Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. |
| Protein Name | Collagen alpha-1(I) chain |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I(1192-1207aa QPPQEKSQDGGRYYRA), identical to the related rat sequence, and different from the related human sequence by two amino acids. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the fibrillar collagen family. |
| Name | Col1a1 {ECO:0000312|MGI:MGI:88467} |
|---|---|
| Function | Type I collagen is a member of group I collagen (fibrillar forming collagen). |
| Cellular Location | Secreted, extracellular space, extracellular matrix {ECO:0000255|PROSITE-ProRule:PRU00793} |
| Tissue Location | Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite |
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Provided below are standard protocols that you may find useful for product applications.
Background
Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.