Anti-Histone H4 (MonoMethyl-K16) Antibody

Referentie AP61437

Formaat : 100ul

Merk : Abcepta

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  • WB - Anti-Histone H4 (MonoMethyl-K16) Antibody AP61437
    Western blot analysis of Histone H4 (MonoMethyl-K16) expression in DLD (A), LOVO (B), U2OS (C) whole cell lysates.
  • E - Anti-Histone H4 (MonoMethyl-K16) Antibody AP61437
    Direct ELISA antibody dose-response curve using Anti-Histone H4 (MonoMethyl-K16) Antibody. Antigen (methyl-peptide and non-methyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P62805
Other Accession P62806
Reactivity Human, Mouse, Rat, Pig, Chicken, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 11367 Da
Additional Information
Gene ID 121504;554313;8294;8359;8360;8361;8362;8363;8364;8365;8366;8367;8368;8370
Other Names H4/A; H4FA; H4/I; H4FI; H4/G; H4FG; H4/B; H4FB; H4/J; H4FJ; H4/C; H4FC; H4/H; H4FH; H4/M; H4FM; H4/E; H4FE; H4/D; H4FD; H4/K; H4FK; H4/N; H4F2; H4FN; HIST2H4; H4/O; H4FO; Histone H4
Target/Specificity Recognizes endogenous levels of Histone H4 with a site at MonoMethyl-K16 protein.
Dilution WB~~WB (1/500 - 1/1000)
E~~WB (1/500 - 1/1000)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name H4C1
Synonyms H4/A, H4FA, HIST1H4A
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H4 with a site at MonoMethyl-K16. The exact sequence is proprietary.

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Cat# AP61437