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> Anti-Histone H4 (MonoMethyl-R19) Antibody

Anti-Histone H4 (MonoMethyl-R19) Antibody

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Referentie AP61438

Formaat : 100ul

Merk : Abcepta

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  • WB - Anti-Histone H4 (MonoMethyl-R19) Antibody AP61438
    Western blot analysis of Histone H4 (MonoMethyl-R19) expression in A375 (A), H446 (B), U2OS (C), mouse kidney (D), rat kidney (E) whole cell lysates.
  • - Anti-Histone H4 (MonoMethyl-R19) Antibody AP61438
    Immunohistochemical analysis of Histone H4 (MonoMethyl-R19) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P62805
Other Accession P62806
Reactivity Human, Mouse, Rat, Pig, Chicken, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 11367 Da
Additional Information
Gene ID 121504;554313;8294;8359;8360;8361;8362;8363;8364;8365;8366;8367;8368;8370
Other Names H4/A; H4FA; H4/I; H4FI; H4/G; H4FG; H4/B; H4FB; H4/J; H4FJ; H4/C; H4FC; H4/H; H4FH; H4/M; H4FM; H4/E; H4FE; H4/D; H4FD; H4/K; H4FK; H4/N; H4F2; H4FN; HIST2H4; H4/O; H4FO; Histone H4
Target/Specificity Recognizes endogenous levels of Histone H4 with a site at MonoMethyl-R19 protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/50 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name H4C1
Synonyms H4/A, H4FA, HIST1H4A
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
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Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol
Blocking Peptides Protocol
Dot Blot Protocol
Immunohistochemistry Protocol
Immunofluorescence Protocol
Immunoprecipitation Protocol
Flow Cytomety Protocol
Cell Culture Protocol

Background

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H4 with a site at MonoMethyl-R19. The exact sequence is proprietary.

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Cat# AP61438