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Reagentia en instrumenten voor immunologie, celbiologie en moleculaire biologie.

Anti-IDH1 antibody (100-200) (Clone: S4MR)

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Referentie NB-22-48673-100

Formaat : 100uL

Neem contact op met een lokale distributeur :

Telefoonnummer : +1 850 650 7790

Merk : Neo Biotech

Meer informatie aanvragen

Neem contact op met een lokale distributeur :

Telefoonnummer : +1 850 650 7790

Western blot analysis of various lysates, using IDH1 rabbit monoclonal antibody at 1:10000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human kidney using IDH1 rabbit monoclonal antibody at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded human testis using IDH1 rabbit monoclonal antibody at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using IDH1 rabbit monoclonal antibody at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat kidney using IDH1 rabbit monoclonal antibody at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Confocal imaging of NIH/3T3 cells using IDH1 rabbit monoclonal antibody (Red). The cells were counterstained with Alpha-Tubulin (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x

General Info

Host:	Rabbit
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit monoclonal antibody anti-IDH1 (100-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Monoclonal
Clone ID:	S4MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:2000-1:10000 IHC-P 1:500-1:1000 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	IDH1
Gene ID:	3417
Uniprot ID:	IDHC_HUMAN
Immunogen Region:	100-200
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100-200 of human IDH1 (NP_005887.2).
Immunogen Sequence:	RNILGGTVFREAIICKNIPR LVSGWVKPIIIGRHAYGDQY RATDFVVPGPGKVEITYTPS DGTQKVTYLVHNFEEGGGVA MGMYNQDKSIEDFAHSSFQM A

Description

Post Translational Modifications	Acetylation at Lys-374 dramatically reduces catalytic activity.
Function	Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase. Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways. May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency.
Protein Name	Isocitrate Dehydrogenase Nadp Cytoplasmic Idh Idh1 Cytosolic Nadp-Isocitrate Dehydrogenase Idpc Nadp(+-Specific Icdh Oxalosuccinate Decarboxylase
Database Links	Reactome: R-HSA-2978092 Reactome: R-HSA-389542 Reactome: R-HSA-6798695 Reactome: R-HSA-9033241 Reactome: R-HSA-9818025
Cellular Localisation	Cytoplasm Cytosol Peroxisome
Alternative Antibody Names	Anti-Isocitrate Dehydrogenase Nadp Cytoplasmic antibody Anti-Idh antibody Anti-Idh1 antibody Anti-Cytosolic Nadp-Isocitrate Dehydrogenase antibody Anti-Idpc antibody Anti-Nadp(+-Specific Icdh antibody Anti-Oxalosuccinate Decarboxylase antibody Anti-IDH1 antibody Anti-PICD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance