Anti-KIT antibody (915-965) {Biotin}

Clonality:	Polyclonal
Conjugation:	Biotin
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.55-0.75 µg/µl
Dilution Range:	WB: 1:500 ELISA: 1:10, 000 IP: 1:150
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	KIT
Gene ID:	3815
Uniprot ID:	KIT_HUMAN
Immunogen Region:	915-965
Immunogen:	Synthetic peptide taken within amino acid region 915-965 and 950-976 on human Tyrosine-Kinase c-Kit protein.

Tissue Specificity	Isoform 3: In testis, detected in spermatogonia in the basal layer and in interstitial Leydig cells but not in Sertoli cells or spermatocytes inside the seminiferous tubules (at protein level). Expression is maintained in ejaculated spermatozoa (at protein level).
Post Translational Modifications	Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation. Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.
Function	Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
Protein Name	Mast/Stem Cell Growth Factor Receptor Kit Scfr Piebald Trait Protein Pbt Proto-Oncogene C-Kit Tyrosine-Protein Kinase Kit P145 C-Kit V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog Cd Antigen Cd117
Database Links	Reactome: R-HSA-1257604 Reactome: R-HSA-1433557 Reactome: R-HSA-1433559 Reactome: R-HSA-2219530 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558 Reactome: R-HSA-8866910 Reactome: R-HSA-9669914 Reactome: R-HSA-9669917 Reactome: R-HSA-9669921 Reactome: R-HSA-9669924 Reactome: R-HSA-9669926 Reactome: R-HSA-9669929 Reactome: R-HSA-9669933 Reactome: R-HSA-9669934 Reactome: R-HSA-9669935 Reactome: R-HSA-9669936 Reactome: R-HSA-9670439 Reactome: R-HSA-9680187
Cellular Localisation	Isoform 1: Cell Membrane Single-Pass Type I Membrane Protein Isoform 2: Cell Membrane Isoform 3: Cytoplasm Detected In The Cytoplasm Of Spermatozoa Especially In The Equatorial And Subacrosomal Region Of The Sperm Head
Alternative Antibody Names	Anti-Mast/Stem Cell Growth Factor Receptor Kit antibody Anti-Scfr antibody Anti-Piebald Trait Protein antibody Anti-Pbt antibody Anti-Proto-Oncogene C-Kit antibody Anti-Tyrosine-Protein Kinase Kit antibody Anti-P145 C-Kit antibody Anti-V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog antibody Anti-Cd Antigen Cd117 antibody Anti-KIT antibody Anti-SCFR antibody

Information sourced from Uniprot.org

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