Anti-MKI67 antibody (1250-1300) {Biotin}

Clonality:	Polyclonal
Conjugation:	Biotin
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.55 µg/µl
Dilution Range:	WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:200 IHC: 1:50-1:150
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	MKI67
Gene ID:	4288
Uniprot ID:	KI67_HUMAN
Immunogen Region:	1250-1300
Specificity:	This antibody will detect both isoform 1 and isoform 2 in human.
Immunogen:	Synthetic peptide taken within amino acid region 1250-1300 on human antigen Ki67 isoform 1.

Post Translational Modifications	Phosphorylated. Hyperphosphorylated in mitosis. Hyperphosphorylated form does not bind DNA.
Function	Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Protein Name	Proliferation Marker Protein Ki-67 Antigen Identified By Monoclonal Antibody Ki-67 Antigen Ki-67 Antigen Ki67
Cellular Localisation	Chromosome Nucleus Nucleolus Associates With The Surface Of The Mitotic Chromosome The Perichromosomal Layer And Covers A Substantial Fraction Of The Mitotic Chromosome Surface Associates With Satellite Dna In G1 Phase Binds Tightly To Chromatin In Interphase Chromatin-Binding Decreases In Mitosis When It Associates With The Surface Of The Condensed Chromosomes Predominantly Localized In The G1 Phase In The Perinucleolar Region In The Later Phases It Is Also Detected Throughout The Nuclear Interior Being Predominantly Localized In The Nuclear Matrix
Alternative Antibody Names	Anti-Proliferation Marker Protein Ki-67 antibody Anti-Antigen Identified By Monoclonal Antibody Ki-67 antibody Anti-Antigen Ki-67 antibody Anti-Antigen Ki67 antibody Anti-MKI67 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance