General Info
| Host: | Rabbit |
| Applications: | WB/IHC/IF |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-OGDH (148-427) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:100-1:500IHC-P 1:50-1:200IF/ICC 1:10-1:100 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Information
| Gene Symbol: | OGDH |
| Gene ID: | 4967 |
| Uniprot ID: | ODO1_HUMAN |
| Immunogen Region: | 148-427 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 148-427 of human OGDH (NP_001003941.1). |
| Immunogen Sequence: | DPLGILDADLDSSVPADIIS STDKLGFYGLDESDLDKVFH LPTTTFIGGQESALPLREII RRLEMAYCQHIGVEFMFIND LEQCQWIRQKFETPGIMQFT NEEKRTLLARLVRSTRFEEF LQRKWSSEKRFGLEGCEVLI PALKTIIDKSSENGVDYVIM GMPHRGRLNVLANVIRKELE QIFCQFDSKLEAADEGSGDV KYHLGMYHRRINRVTDRNIT LSLVANPSHLEAADPVVMG |
Description
| Function | 2-oxoglutarate dehydrogenase (E1o) component of the 2-oxoglutarate dehydrogenase complex (OGDHC). Participates in the first step, rate limiting for the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2) catalyzed by the whole OGDHC. Catalyzes the irreversible decarboxylation of 2-oxoglutarate (alpha-ketoglutarate) via the thiamine diphosphate (ThDP) cofactor and subsequent transfer of the decarboxylated acyl intermediate on an oxidized dihydrolipoyl group that is covalently amidated to the E2 enzyme (dihydrolipoyllysine-residue succinyltransferase or DLST). Plays a key role in the Krebs (citric acid) cycle, which is a common pathway for oxidation of fuel molecules, including carbohydrates, fatty acids, and amino acids. Can catalyze the decarboxylation of 2-oxoadipate in vitro, but at a much lower rate than 2-oxoglutarate. Mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A. |
| Protein Name | 2-Oxoglutarate Dehydrogenase Complex Component E1E1oOgdc-E1Ogdh-E12-Oxoglutarate Dehydrogenase - MitochondrialAlpha-Ketoglutarate DehydrogenaseAlpha-Kgdh-E1Thiamine DiphosphateThdp-Dependent 2-Oxoglutarate Dehydrogenase |
| Database Links | Reactome: R-HSA-389661Reactome: R-HSA-71064Reactome: R-HSA-71403 |
| Cellular Localisation | MitochondrionNucleusMainly Localizes In The MitochondrionA Small Fraction Localizes To The NucleusWhere The 2-Oxoglutarate Dehydrogenase Complex Is Required For Histone Succinylation |
| Alternative Antibody Names | Anti-2-Oxoglutarate Dehydrogenase Complex Component E1 antibodyAnti-E1o antibodyAnti-Ogdc-E1 antibodyAnti-Ogdh-E1 antibodyAnti-2-Oxoglutarate Dehydrogenase - Mitochondrial antibodyAnti-Alpha-Ketoglutarate Dehydrogenase antibodyAnti-Alpha-Kgdh-E1 antibodyAnti-Thiamine Diphosphate antibodyAnti-Thdp-Dependent 2-Oxoglutarate Dehydrogenase antibodyAnti-OGDH antibody |
Information sourced from Uniprot.org
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