Anti-Tubulin alpha Monoclonal Antibody
Referentie OAAI00288
Formaat : 100ug
Merk : Aviva Systems Biology
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Telefoonnummer : +1 850 650 7790
Anti-Tubulin alpha Monoclonal Antibody (OAAI00288)
| Datasheets/Manuals | Printable datasheet for Anti-Tubulin alpha Monoclonal Antibody (OAAI00288) |
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| Predicted Species Reactivity | Human|Mouse|Rat |
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| Product Format | Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
| Clonality | Monoclonal |
| Clone | ABM359 |
| Isotype | IgG1 |
| Host | Mouse |
| Application | ELISA, FC, IHC, IP, WB |
| Additional Information | Background: Tubulin is the major building block of microtubules which is essential in cell structures, transportation and mitosis. This superfamily is composed of six distinct families. Alpha, beta, and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. Each subunit is a 50 kDa acidic protein. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. |
| :: | Positive Control: Hela cells, Lung tissue |
| :: | Cellular Location: Cytoplasmic |
| Reconstitution and Storage | The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles. |
| Immunogen | A recombinant protein of human Tubulin. |
| Purification | The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping |
| Concentration | 100ug/200ul |
| Specificity | TUBA1A |
| Formulation | This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
| Application Info | WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested |
| Reference | Ravindra, R : Aronstam, R S Influence of anti-tubulin antibodies on muscarinic receptor modulation of G protein GTPase activity in Rat striatum. Biochem-Pharmacol. 1990 Aμg 1; 40(3): 457-63 |
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| Gene Symbol | AVSD1 |
| Gene Full Name | atrioventricular septal defect 1 |
| Alias Symbols | AVCD;AVCD1;AVSD;ventricular septal defect 1;VSD1. |
| NCBI Gene Id | 7446 |
| Description of Target | Tubulin is the major building block of microtubules which is essential in cell structures, transportation and mitosis. This superfamily is composed of six distinct families. Alpha, beta, and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. Each subunit is a 50 kDa acidic protein. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. |
| Molecular Weight | 50 |
