AP chromogen BCIP/NBT (ready-to-use)
Referentie GTX27468-100ml
Formaat : 100ml
Merk : Genetex
Neem contact op met een lokale distributeur :
Telefoonnummer : +1 850 650 7790
Application | WB, IHC-P, IHC-Fr, Dot |
|---|---|
| Package | 100 ml |
Datasheet File View product citations for antibody GTX27468 on CiteAb
PRODUCT
Summary
Alkaline Phosphatase chromogen BCIP/NBT (ready-to-use)
APPLICATION
Application Note
See ProtocolBCIP/NBT system is based on hydrolysis of BCIP and reduction of NBT producing a deep purple reaction product. Reaction is several times more sensitive than existing products with little or no background staining. The reaction product is extremely stable and does not fade when exposed to light.
PROPERTIES
Form
Liquid
Storage
Discard if the solution turns purple or turbid. The reagent is stable at room temperature, but we recommend storage in 2ºC to 8ºC to increase the shelf life. Warm to assay temperature before use.
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BCIP NBT
Background
Alkaline Phosphatase based immunostaining systems are gaining wide acceptance among pathologists because of their relatively high signal to noise ratio. 5-bromo,4-chloro,3-indolylphosphate (BCIP)/nitroblue tetrazolium (NBT) substrate for alkaline phosphatase-based detection systems, is a commonly used substrate chromogen. BCIP acts as the substrate for alkaline phosphatase, and the NBT enhances the purplish-brown final color of the precipitates at the reaction sites.
Application Reference
Moser K et al. Arthritis Rheum 2012; 64 (4) : 1237-46 CXCR3 promotes the production of IgG1 autoantibodies but is not essential for the development of lupus nephritis in NZB/NZW mice.
REVIEW
Application Reference
Moser K et al. Arthritis Rheum 2012; 64 (4):1237-46 CXCR3 promotes the production of IgG1 autoantibodies but is not essential for the development of lupus nephritis in NZB/NZW mice.
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