Aspartate Aminotransferase (AST/GOT) Assay Kit

Referentie abx096003-96tests

Formaat : 96tests

Merk : Abbexa

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Aspartate Transaminase (AST / GOT) Assay Kit is an assay kit designed for the detection and quantification of Aspartate Transaminase activity in serum, plasma, tissue homogenates, cell lysates and cell culture supernatants using a microplate reader (Reitman-Frankel Method). AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine (DNPH) to form 2,4-dinitrophenylhydrazone showing reddish-brown in alkaline solution. Measure the OD values and calculate the enzyme activity. This kit is also available for the Spectrophotometric detection - abx294136.

Target Aspartate Aminotransferase (AST/GOT)
Storage Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity The validity for this kit is 12 months.
Buffer Not applicable.
Test Range 1.1 IU/L - 72.3 IU/L
Sensitivity 1.1 IU/L
Detection Method Colorimetric
Assay Data Quantitative
CAS Number 9000-97-9
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Aspartate Aminotransferase (AST/GOT)