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Left: Western blot analysis of Aurora A/B/C kinase in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with Aurora A/B/C. The antibody monoclonal binds strongly to bands at about 46 kDa and 38kDa, corresponding to Aurora A and Aurora B. Also, it recognizes a weak band at 35 kDa which is Aurora C. Right: Blot of recombinant full length human Aurora A, B and C was probed with MO22139. This antibody reacts strongly with all three Aurora kinases proteins.
Left: Western blot analysis of Aurora A/B/C kinase in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with Aurora A/B/C. The antibody monoclonal binds strongly to bands at about 46 kDa and 38kDa, corresponding to Aurora A and Aurora B. Also, it recognizes a weak band at 35 kDa which is Aurora C. Right: Blot of recombinant full length human Aurora A, B and C was probed with MO22139. This antibody reacts strongly with all three Aurora kinases proteins.
HeLa cell cultures were stained with Aurora A/B/C kinase antibody (green). In this image, strong staining in spindle poles was seen in cells at anaphase. This antibody also stains the midbodies between the two daughter cells. Counterstained is our chicken polyclonal antibody against Vimentin, (red, CH22108). Blue is a DNA stain.
HeLa cell cultures were stained with Aurora A/B/C kinase antibody (green). In this image, strong staining in spindle poles was seen in cells at anaphase. This antibody also stains the midbodies between the two daughter cells. Counterstained is our chicken polyclonal antibody against Vimentin, (red, CH22108). Blue is a DNA stain.