Avidin antibody (Peroxidase)
Referentie orb344921-20mg
Formaat : 20mg
Merk : Biorbyt
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Avidin antibody (Peroxidase)
Catalog Number: orb344921
| Catalog Number | orb344921 |
|---|---|
| Category | Antibodies |
| Description | Avidin antibody (Peroxidase) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | DOT, ELISA, IHC, WB |
| Reactivity | Bacteria |
| Isotype | IgG |
| Immunogen | Avidin (Hen Egg White) |
| Concentration | 10.0 mg/mL |
| Dilution range | ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,000, WB: 1:2,000 - 1:5,000 |
| Form/Appearance | Lyophilized |
| Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White ]. Cross reactivity against Avidin from other sources is unknown. |
| Conjugation | HRP |
| UniProt ID | P02701 |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Alternative names | rabbit anti-Avidin Antibody Peroxidase Conjugation |
| Note | For research use only |
| Application notes | Anti-Avidin Antibody Peroxidase Conjugated has been tested in WB and assayed against 1.0 ug of Avidin [Hen Egg White ] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:27,000 of the reconstitution concentration is suggested for this product. This product can be suitable for ELISA, Western Blot, and Immunohistochemistry. |
| Expiration Date | 12 months from date of receipt. |
Western Blot of Rabbit Anti-Avidin Antibody Peroxidase Conjugated. Lane 1: Molecular Weight Marker. Lane 2: Avidin [0.05 µg]. Lane 3: Streptavidin [0.05 µg]. Primary Antibody: Anti-Avidin HRP at 1.0 µg/mL overnight at 2-8°C. Secondary Antibody: none. Blocking Buffer: BlockOut (p/n orb348644) for 30 mins at RT. Predicted MW: ~17 kDa.