BCIP/NBT chromogen/substrate
Referentie GTX73326-15ml
Formaat : 15ml
Merk : GeneTex
Neem contact op met een lokale distributeur :
Telefoonnummer : +1 850 650 7790
Application | IHC |
|---|---|
| Package | 15 ml |
Datasheet File View product citations for antibody GTX73326 on CiteAb
PRODUCT
Summary
Alkaline Phosphatase Substrate-Chromogen (BCIP/NBT)
APPLICATION
Application Note
1. Once tissue sections have been incubated with alkaline phosphatase, wash them with buffer thoroughly. 2. Wipe the glass to remove excess of buffer and add enough drops of the BCIP/NBT solution to cover the tissue sections. 3. Incubate for 10-30 minutes at room temperature. For the best results, look under the microscope for the signal development. Once desired signal to noise ratio is achieved, stop the reaction by washing the slides in the wash buffer.
PROPERTIES
Storage
Keep as concentrated solution, aliquot and store at 4ºC. DO NOT FREEZE.
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Background
Alkaline phosphatase, an enzyme derived from bovine intestinal mucosa, is often used as a label for in situ hybridization, immunohistochemistry, Southern and Northern hybridization and DNA sequencing. Detection of this enzyme requires the use of an insoluble colored substrate. GTX73326 is a new stable, BCIP/NBT solution in ready to use form. It produces a blue colored end product at the positive sites which gives a good contrast with red nuclear fast counter stain. Specimens stained in BCIP/NBT can not be dehydrated in ethanol and hence should be mounted in aqueous based mounting medium.