31-1042-02 | Biotin Anti-BRAF (V600E) rabbit monoclonal antibody

Product Details
Product Name	BRAF V600E Rabbit Monoclonal Antibody, Clone RM8
Product Description	Rabbit monoclonal to BRAF V600E Mutant, BRAF(V600E), BRAF-V600E
Catalog No.	31-1042-00; 31-1042-00-S; 31-1042-02
Clone Name	RM8
Product Data Sheet	BRAF V600E Rabbit Monoclonal Antibody Data Sheet Biotinylated BRAF V600E Rabbit Monoclonal Antibody Data Sheet
Specificity	RM8 reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF.
Immunogen	A peptide corresponding to BRAF V600E mutant
Application	Immunohistochemistry Immunocytochemistry Western Blot ELISA Flow Cytometry
Protocols	BRAF V600E IHC Protocol
Species Reactivity	Human
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size	100 µg; 50 µg; Biotinylated 50 µg
Concentration	1 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Immunohistochemistry (IHC): 0.5 µg/mL – 5 µg/mL; Immunocytochemistry (ICC): 0.5 µg/mL – 5 µg/mL; Western Blot (WB): 0.5 µg/mL – 2 µg/mL; ELISA: 0.5 µg/mL – 2 µg/mL;
References for BRAF V600E Mutant antibody [RM8]	Labib M et al. Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting. Nat Biomed Eng. (2020), 10.1038/s41551-020-0590-1. FC; Human. Read more (PubMed: 32719513) Crespo-Rodriguez E et al. Combining BRAF inhibition with oncolytic herpes simplex virus enhances the immune-mediated antitumor therapy of BRAF-mutant thyroid cancer. J Immunother Cancer. (2020), 10.1136/jitc-2020-000698. WB; Human. Read more (PubMed: 32759235) Martin AM et al. PD-L1 Expression in Pediatric Low-Grade Gliomas Is Independent of BRAF V600E Mutational Status. J Neuropathol Exp Neurol. (2020), 10.1093/jnen/nlz119. WB; Human. Read more (PubMed: 31819973) Shirsat PM et al. Low frequency of BRAF V600E immunoexpression in mandibular ameloblastomas: An institutional study. J Oral Maxillofac Pathol. (2018), 10.4103/jomfp.JOMFP_174_17. IHC; Human. Read more (PubMed: 30651680) Usman H et al. Result Analysis of BRAF V600E Gene Mutation Using Molecular and Immunohistochemistry Detection in Acral Malignant Melanoma. Asian Pacific Journal of Cancer Care. (2018), 10.31557/APJCC.2018.3.3.43. IHC; Human. Read more (WAOCP) Hansen C et al. Nanoswitch-linked immunosorbent assay (NLISA) for fast, sensitive, and specific protein detection. Proc Natl Acad Sci USA. (2017), 10.1073/pnas.1708148114. ELISA, WB; Human. Read more (PubMed: 28893984) Krishnamurthy A et al. Clinical utility of immunohistochemistry using the novel anti-BRAF V600E antibody (clone RM8) for detection of the BRAF V600E mutant protein in papillary thyroid cancers. Int J Mol ImmunoOncol. (2018) 10.18203/issn.2456-3994.IntJMolImmunoOncol20180471. IHC-P; Human. Read more (IJMIO: ISSN: 2456-3994) Gouravan S et al. Preclinical Evaluation of Vemurafenib as Therapy for BRAFV600E Mutated Sarcomas. Int J Mol Sci. (2018) 10.3390/ijms19040969. WB; Human. Read more (PubMed: 29570692) Yang H et al. Activated MEK cooperates with Cdkn2a and Pten loss to promote the development and maintenance of melanoma. Oncogene (2017) 10.1038/onc.2016.526. WB; Human. Read more (PubMed: 28263969) ZM Shaban et al. BRAFV600E Mutation in Gliomas in Iraqi Patients Immunohistochemical Study. ijcocr (2017) 10.11648/j.ijcocr.20170206.11. IHC; Human. Read More
Product Images
	Immunohistochemical staining of formalin fixed and paraffin embedded melanoma tissue sections using anti-BRAF V600E rabbit monoclonal antibody, Clone RM8, at 2 µg/mL.
	Immunohistochemical staining of formalin fixed and paraffin embedded Thyroid Carcinoma tissue using anti-BRAF V600E rabbit monoclonal antibody, Clone RM8, at 0.5 µg/mL. Allele specific PCR validated to be Negative for BRAF V600E.
	Immunohistochemical staining of formalin fixed and paraffin embedded Thyroid Carcinoma tissue using anti-BRAF V600E rabbit monoclonal antibody, Clone RM8, at 0.5 µg/mL. Allele specific PCR validated to be Positive for BRAF V600E.
	Western Blot analysis of A375 cell (BRAF V600E) and HEK293 cell (WT) lysates using anti-BRAF V600E rabbit monoclonal antibody, Clone RM8, at 2 µg/mL.
	Immunohistochemical staining of formalin fixed and paraffin embedded human cell line sections, A375 Cells (Positive), at 1 µg/mL.
	Immunohistochemical staining of formalin fixed and paraffin embedded human cell line sections, Hela Cells (Negative), at 1 µg/mL.

Biotin Anti-BRAF (V600E) rabbit monoclonal antibody [RM8]

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