Biotin Glucose Oxidase Conjugated
Referentie OORA00148
Formaat : 5mg
Merk : Aviva Systems Biology
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Biotin Glucose Oxidase Conjugated (OORA00148)
| Datasheets/Manuals | Printable datasheet for OORA00148 |
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| Product Format | Lyophilized 0.01M Sodium Phosphate, 0.15M Sodium Chloride (pH 7.2) |
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| Conjugation | Biotin |
| Application | DB, ELISA, IHC, WB |
| Additional Information | Conjugation Chemistry: N-hydroxtsuccinimide (NHS) ester. |
| Reconstitution and Storage | Restore with 5.0 mL deionized water (or equivalent). Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product is not completely clear after standing at room temperature. Expiration date is one year from date of receipt. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. |
| Concentration | 1 mg/ml |
| Specificity | Biotin Glucose Oxidase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Glucose Oxidase and anti-Biotin. |
| Application Info | Biotin Glucose Oxidase Conjugated can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin. All assays should be optimized by the user. Recommended dilutions are listed below: ELISA:1:8,000 - 1:32,000 WB: 1:500 - 1:2,500 IHC: 1:200 - 1:1,000 |
| Purification | Biotin Glucose Oxidase Conjugated was prepared from chromatographically purified biotin. |
| Alias Symbols | Biotin Glucose Oxidase Conjugation, Glucose Oxidase Conjugated Biotin, Biotin conjugated Glucose Oxidase |
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| Description of Target | Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, Biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin; an attraction that is the strongest and most stable non-covalent interaction known. Glucose oxidase is an enzyme that drives the oxidation of glucose into hydrogen peroxide and D-glucono-d-lactone. This activity is utilized in biotechnology through colormetric detection assays that are sensitive to the amount of hydrogen peroxide produced. Biotin Glucose Oxidase Conjugated is ideal for investigators involved in Cancer, Cell Signaling, Neuroscience, and Cell Biology research. |
| Molecular Weight | 455 |