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> Bromoacetamido-PEG2-C2-NHS ester [1353011-78-5]

Bromoacetamido-PEG2-C2-NHS ester [1353011-78-5]

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Referentie HY-141386-50mg

Formaat : 50mg

Merk : MedChemExpress

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Bromoacetamido-PEG2-C2-NHS ester is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs.

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Bromoacetamido-PEG2-C2-NHS ester Chemical Structure

Bromoacetamido-PEG2-C2-NHS ester Chemical Structure

CAS No. : 1353011-78-5

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

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PEG Linkers Alkyl-Chain Linkers Alkyl/ether Linkers
Description

Bromoacetamido-PEG2-C2-NHS ester is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1].

IC50 & Target

PEGs

 

Alkyl/ether

 

In Vitro

PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Bromoacetamido-PEG2-C2-NHS ester Related Antibodies
Masse moléculaire

395.20

Formule

C13H19BrN2O7
CAS No.

1353011-78-5
Appearance

Solid-Liquid Mixture

Color

White to off-white

SMILES

O=C(ON1C(CCC1=O)=O)CCOCCOCCNC(CBr)=O
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage
Pure form -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvant et solubilité
In Vitro: 

DMSO : 100 mg/mL (253.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5304 mL 12.6518 mL 25.3036 mL
5 mM 0.5061 mL 2.5304 mL 5.0607 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.33 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.33 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation

Purity: ≥95.0%

COA (244 KB) HNMR (255 KB) MS (69 KB)

Instruction de manipulation (2659 KB)
Références

  • [1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562  [Content Brief]

  • [1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5304 mL 12.6518 mL 25.3036 mL 63.2591 mL
5 mM 0.5061 mL 2.5304 mL 5.0607 mL 12.6518 mL
10 mM 0.2530 mL 1.2652 mL 2.5304 mL 6.3259 mL
15 mM 0.1687 mL 0.8435 mL 1.6869 mL 4.2173 mL
20 mM 0.1265 mL 0.6326 mL 1.2652 mL 3.1630 mL
25 mM 0.1012 mL 0.5061 mL 1.0121 mL 2.5304 mL
30 mM 0.0843 mL 0.4217 mL 0.8435 mL 2.1086 mL
40 mM 0.0633 mL 0.3163 mL 0.6326 mL 1.5815 mL
50 mM 0.0506 mL 0.2530 mL 0.5061 mL 1.2652 mL
60 mM 0.0422 mL 0.2109 mL 0.4217 mL 1.0543 mL
80 mM 0.0316 mL 0.1581 mL 0.3163 mL 0.7907 mL
100 mM 0.0253 mL 0.1265 mL 0.2530 mL 0.6326 mL
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Bromoacetamido-PEG2-C2-NHS ester Related Classifications

  • PROTAC
  • PROTAC Linkers
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bromoacetamido-PEG2-C2-NHS ester1353011-78-5PROTAC LinkersInhibitorinhibitorinhibit

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