Sequence:|5'-G T A^T A C-3' |3'-C A T^A T G-5'||Source: |Bacillus stearothermophilus RFL1107||Concentration: |10u/ul||Unit Definition:|One unit is defined as the amount of Bst1107I is required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of recommended reaction buffer.||Form:|Supplied as a liquid in 10mM Tris-HCl pH 7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.||Supplied with:|R1625: Restriction Enzyme Buffer A, 10X: |Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9 at 37°C, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.||R1625-03: Restriction Enzyme Buffer D, 10X: |Dilute to 1X for use. 1X buffer composition is 50mM Tris-HCl, pH 7.5, 10mM MgCl2, 100mM sodium chloride, 0.1mg/ml BSA.||Thermal Inactivation: |Enzyme is inactivated by incubation at 80°C for 20 minutes. ||Diluent Buffer:|10mM Tris-HCl pH 7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.||Enzyme Properties:|Methylation Effects:|Dam, Never overlaps - no effect|Dcm: Never overlaps - no effect|CpG: May overlap - cleavage impaired|EcoKI: Never overlaps - no effect|EcoBI: Never overlaps - no effect||Stability during Prolonged Incubation: |A minimum of 0.1units of Bst1107I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C. ||Digestion of Agarose-embedded DNA: |A minimum of 5 units is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours.||Number of Recognition Sites in DNA: |Lambda: 3|PhiX174: 0|M13mp18/19: 0|pBR322: 1|pUC18/19: 0|pUC57: 0|pTZ19R/U: 0||Overdigestion Assay:|No detectable change in the specific fragmentation pattern is observed after a 160-fold overdigestion with Bst1107I (10U/ug lambda DNA x 16 hours).||Ligation and Recleavage (L/R) Assay:|The ligation and recleavage assay was replaced with LO test after validating experiments showed LO test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100.||Labeled Oligonucleotide (LO) Assay:|No detectable degradation of single-stranded or double- stranded labeled oligonucleotides occurred during incubation with 10 units of Bst1107I for 4 hours.||Blue/White Cloning Assay: |The B/W assay was replaced with L0 test after validating experiments showed L0 test ability to detect nuclease and phophatase activities with sensitivity that equals to that of B/W test.||Storage and Stability:|May be stored at 4°C. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.