B7755-1mg | Calcein AM (Calcein acetoxymethyl ester) [148504-34-1

Background

Calcein AM (calcein acetoxymethyl ester) is a non-fluorescent, uncharged molecule, which is freely permeable to the plasma membrane of viable cells. Upon entering live cells, calcein AM is cleaved by nonspecific esterases in the cytoplasm to form membrane-impermeant calcein, a fluorescent indicator with absorption and emission maxima of 494 and 517 nm, respectively. Calcein AM has wide-ranging applications in the fields of cell viability, cell-cell communication and cytotoxicity. In addition, calcein is a substrate for multidrug resistance-associated protein 1 (MRP1) and multidrug resistance protein 3 (MDR3, P-glycoprotein 3), which makes calcein AM a useful tool for studying these proteins and their regulation.

References:

1. Miles FL, Lynch JE, Sikes RA. Cell-based assays using calcein acetoxymethyl ester show variation in fluorescence with treatment conditions. Journal of Biological Methods, 2015, 2(3): e29.

2. Liu X, Zhu XY, Chen M, et al. Resveratrol protects PC12 cells against OGD/R-induced apoptosis via the mitochondrial-mediated signaling pathway. Acta Biochimica et Biophysica Sinica, 2016, 48(4): 342-353.

3. Polli JW, Wring SA, Humphreys JE, et al. Rational use of in vitro P-glycoprotein assays in drug discovery. Journal of Pharmacology and Experimental Therapeutics, 2001, 299(2): 620-628.

4. Tiberghien F, Loor F. Ranking of P-glycoprotein substrates and inhibitors by a calcein-AM fluorometry screening assay. Anticancer Drugs, 1996, 7(5): 568-578.

5. Szakács G, Jakab K, Antal F, et al. Diagnostics of multidrug resistance in cancer. Pathology & Oncology Research, 1998, 4(4): 251-257.

Product Citation

1. Yin Ni, Bang-Chuan Hu, et al. "Interruption of neutrophil extracellular traps formation dictates host defense and tubular HOXA5 stability to augment efficacy of anti-Fn14 therapy against septic AKI." Theranostics. 2021 Sep 13;11(19):9431-9451. PMID: 34646379

Chemical Properties

Physical Appearance	A crystalline solid
Storage	Store at -20°C
M.Wt	994.86
Cas No.	148504-34-1
Formula	C₄₆H₄₆N₂O₂₃
Solubility	Soluble in DMSO
Chemical Name	tetrakis(acetoxymethyl) 2,2',2'',2'''-(((3',6'-diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-4',5'-diyl)bis(methylene))bis(azanetriyl))tetraacetate
SDF	Download SDF
Canonical SMILES	O=C1O[C@@]2(C3=CC=CC=C31)C(C=C4)=C(C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C4OC(C)=O)OC5=C2C=CC(OC(C)=O)=C5CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Quality Control

Purity ≥90.00%

COA (Certificate Of Analysis)
MSDS (Material Safety Data Sheet)

Calcein AM (Calcein acetoxymethyl ester) [148504-34-1]

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Background

Product Citation

Chemical Properties

Quality Control

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