K2062-100 assays | Calpain Activity Fluorometric Assay Kit Clinisciences

Background

Apoptosis is one of the biological processes that activation of calpain is implicated in. Cell membrane and Ca²⁺ is required for calpainactivation. The activated calpain is entered into cytosol. The Calpain Activity Assay Kit is a convenient way of measuring calpain activity with optimized buffers and reagents. The Extraction Buffer extracts cytosolic proteins with contaminations from cell membrane and lysosome proteases. It also protects the cell from atuo-activation of calpain during extraction process. Thus, the kit only detects activated calpain in cytosol following treatment of cells with chemicals or drugs inducers. The fluorometric assay is based on the detection of cleavage of calpain substrate Ac-LLY-AFC. Ac-LLY-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by calpain, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorescence.

Features & Properties

Features	Simple one-step procedure; takes only 2 hours. Fast and convenient. The kit contains both positive and negative controls. Active calpain and calpain inhibitor and antibodies are also available separately.
Shipping	Dry ice
Storage Conditions	Store at -70°C.

Quality Control

DataSheet

Calpain Activity Fluorometric Assay Kit

Referentie K2062-100 assays

Neem contact op met een lokale distributeur :

Merk : APExBIO Technology

Neem contact op met een lokale distributeur :

Background

Features & Properties

Quality Control