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Catalase Colorimetric Activity Kit

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Referentie OKAU00112

Formaat : 2plate

Merk : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for Catalase Colorimetric Activity Kit (OKAU00112)
Product Info
ApplicationAA
ELISA Kit Detection MethodColorimetric
ELISA Kit LinearityLinearity was determined by taking two serum samples, one with a high known catalase activity of 1.163 U/ mL and the other with a lower catalase activity of 0.485 U/mL and mixing them in the ratios given below. The measured activities were compared to the expected values based on the ratios used.
High SampleLow SampleExpected Activity (U/mL)Observed Activity (U/mL)% Recovery
80%20%1.0271.058103.0
60%40%0.8920.952106.8
40%60%0.7560.66587.9
20%80%0.6210.59395.6
Mean Recovery98.3%
ELISA Kit PrincipleThe DetectX® Catalase Activity Kit is designed to quantitatively measure catalase activity in a variety of samples. Please read the complete kit insert before performing this assay. A bovine catalase standard is provided to generate a standard curve for the assay and all samples should be read off of the standard curve. Samples are diluted in the provided Assay Buffer and added to the wells of a half area clear plate. Hydrogen peroxide is added to each well and the plate incubated at room temperature for 30 minutes. The supplied Substrate is added, followed by diluted horseradish peroxidase and incubated at room temperature for 15 minutes. The HRP reacts with the substrate in the presence of hydrogen peroxide to convert the colorless substrate into a pink-colored product. The colored product is read at 560 nm. Increasing levels of catalase in the samples causes a decrease in H2O2 concentration and a reduction in pink product. The activity of the catalase in the sample is calculated after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of units of catalase activity per mL.
ELISA Kit ReproducibilityIntra Assay Precision Three human serum samples diluted in Assay Buffer were run in replicates of 20 in an assay. The mean and precision of the calculated concentrations were:
SampleCatalase Activity (U/mL)%CV
11.713.5
20.844.0
30.484.8
Inter Assay Precision Three human serum samples diluted in Assay Buffer were run in duplicates in twenty-one assays run over multiple days by three operators. The mean and precision of the calculated concentrations were:
SampleCatalase Activity (U/mL)%CV
11.7911.9
20.949.8
30.5312.3
ELISA Kit Component
ComponentQuantity
Clear Half Area 96 Well Plates2 Plates
Catalase Standard90 uL
Assay Buffer Concentrate25 mL
Hydrogen Peroxide Reagent5 mL
Substrate5 mL
Horseradish Peroxidase Concentrate120 uL
Additional InformationBackground: Hydrogen peroxide, H2O2 is one of the most frequently occurring reactive oxygen species. It is formed either in the environment or as a by-product of aerobic metabolism, superoxide formation and dismutation, or as a product of oxidase activity. Both excessive hydrogen peroxide and its decomposition product hydroxyl radical, formed in a Fenton-type reaction, are harmful for most cell components. Its rapid removal is essential for all aerobically living prokaryotic and eukaryotic cells. Hydrogen peroxide however can act as a second messenger in signal transduction pathways, in immune cell activation, inflammation processes, cell proliferation, and apoptosis.
::Detection Limit: 0.062 U/mL
Reconstitution and Storage2°C to 8°C
Sample TypeSerum, Plasma, Cells, Tissues and Erythrocyte Lysates
Sensitivity0.052 U/mL
Gene Full NameCatalase Colorimetric