CD133 (PROM1) (NM_001145847) Human 3' UTR Clone

Referentie SC212890

Formaat : 10ug

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CD133 (PROM1) (NM_001145847) Human 3' UTR Clone

SKU
SC212890
3' UTR clone of prominin 1 (PROM1) transcript variant 2 for miRNA target validation
4 Weeks*
Bulk Requests & Clone Modifications
Specifications
Product Data
Vector pMirTarget
Species Human
transfection_reporter RFP
assay_reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Target Symbol CD133
Synonyms AC133; CD133; CORD12; MCDR2; MSTP061; PROML1; RP41; STGD4
ACCN NM_001145847
Insert Size 1195 bp
Sequence Data
Insert Sequence
>SC212890 3’UTR clone of NM_001145847
The sequence shown below is from the reference sequence of NM_001145847. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
CCTGTTATGACAAGCCCATCACAACATTGATAGCTGATGTTGAAACTGCTTGAGCATCAGGATACTCAA
AGTGGAAAGGATCACAGATTTTTGGTAGTTTCTGGGTCTACAAGGACTTTCCAAATCCAGGAGCAACGC
CAGTGGCAACGTAGTGACTCAGGCGGGCACCAAGGCAACGGCACCATTGGTCTCTGGGTAGTGCTTTAA
GAATGAACACAATCACGTTATAGTCCATGGTCCATCACTATTCAAGGATGACTCCCTCCCTTCCTGTCT
ATTTTTGTTTTTTACTTTTTTACACTGAGTTTCTATTTAGACACTACAACATATGGGGTGTTTGTTCCC
ATTGGATGCATTTCTATCAAAACTCTATCAAATGTGATGGCTAGATTCTAACATATTGCCATGTGTGGA
GTGTGCTGAACACACACCAGTTTACAGGAAAGATGCATTTTGTGTACAGTAAACGGTGTATATACCTTT
TGTTACCACAGAGTTTTTTAAACAAATGAGTATTATAGGACTTTCTTCTAAATGAGCTAAATAAGTCAC
CATTGACTTCTTGGTGCTGTTGAAAATAATCCATTTTCACTAAAAGTGTGTGAAACCTACAGCATATTC
TTCACGCAGAGATTTTCATCTATTATACTTTATCAAAGATTGGCCATGTTCCACTTGGAAATGGCATGC
AAAAGCAATCATAGAGAAACCTGCGTAACTCCATCTGACAAATTCAAAAGAGAGAGAGAGATCTTGAGA
GAGAAATGCTGTTCGTTCAAAAGTGGAGTTGTTTTAACAGATGCCAATTACGGTGTACAGTTTAACAGA
GTTTTCTGTTGCATTACGATAAACATTAATTGGAGTGCAGCTAACATGAGTATCATCAGACTAGTATCA
AGTGTTCTAAAATGAAATATGAGAAGATCCTGTCACAATTCTTAGATCTGGTGTCCAGCATGGATGAAA
CCTTTGAGTTTGGTCCCTAAATTTGCATGAAAGCACAAGGTAAATATTCATTTGCTTCAGGAGTTTCAT
GTTGGATCTGTCATTATCAAAAGTGATCAGCAATGAAGAACTGGTCGGACAAAATTTAACGTTGATGTA
ATGAAATTCCAGATGTAGGCATTCCCCCCAGGTCTTTTCATGTGCAGATTGCAGTTCTGATTCATTTGA
ATAAAAAGGAACTTGGAAAACA
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Reference Data
RefSeq NM_001145847.2
Locus ID 8842
Summary This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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