Reagentia en instrumenten voor immunologie, celbiologie en moleculaire biologie.

CD5(Cris-1), 0.2mg/mL

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Referentie BNUB0176-100

Formaat : 100uL

Neem contact op met een lokale distributeur :

Telefoonnummer : +1 850 650 7790

Merk : Biotium

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Neem contact op met een lokale distributeur :

Telefoonnummer : +1 850 650 7790

Antibody number	#0176
Antibody reactivity (target)	CD5
Antibody type	Primary
Host species	Mouse
Clonality	Monoclonal
Clone	Cris-1
Isotype	IgG2a, kappa
Molecular weight	67 kDa
Synonyms	CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5
Human gene symbol	CD5
Entrez gene ID	921
SwissProt	P06127
Unigene	58685
Immunogen	Stimulated human leukocytes
Antibody target cellular localization	Plasma membrane
Verified antibody applications	Flow (verified)
Species reactivity	Human, Rhesus monkey
Expected antibody applications	ELISA (published for clone), Functional studies (published for clone), IP (published for clone)
Antibody application notes	Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Immunofluorescence 0.5-1 ug/mL Immunoprecipitation 0.5-1 ug/500 ug protein lysate, Optimal dilution for a specific application should be determined by user
Positive control	293T, Ramos, or MOLT-4 Cells. Tonsil.
Shipping condition	Room temperature
Storage Conditions	Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life	Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status	For research use only (RUO)
Antibody/conjugate formulation	Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Antibody research areas	Immunology
Product origin	Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Cell/tissue expression	Lymphocytes, T-cells
Tumor expression	Leukemia/lymphoma

Product Description

Recognizes a 67 kDa transmembrane protein, which is identified as CD5 (HLDA I; WS Code T 29HLDA III; WS Code T 530). The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email info@clinisciences.com to inquire about stock status and lead times before placing your order.

Catalog number key for antibody number 0176, Anti-CD5 (Cris-1)

Antibody # prefix	Conjugation	Ex/Em (nm)	Laser line	Detection channel	Dye Features
BNC04	CF®405S	404/431	405	DAPI (microscopy), AF405	CF®405S Features
BNC88	CF®488A	490/515	488	GFP, FITC	CF®488A Features
BNC68	CF®568	562/583	532, 561	RFP, TRITC	CF®568 Features
BNC94	CF®594	593/614	561	Texas Red®	CF®594 Features
BNC40	CF®640R	642/662	633-640	Cy®5	CF®640R Features
BNC47	CF®647	650/665	633-640	Cy®5	CF®647 Features
BNC74	CF®740	742/767	633-685	775/50	CF®740 Features
BNCB	Biotin	N/A	N/A	N/A
BNUB	Purified	N/A	N/A	N/A
BNUM	Purified, BSA-free	N/A	N/A	N/A

Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

Note: Listed references are for this antibody clone sold by Biotium and other suppliers.

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