Flow Cytometry: Neat - 1/10; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivity
Feline
Antibody Host
Mouse
Isotype
IgG1
Clonality
Monoclonal
Specificity
This antibody recognises CD5, a 67kD cell surface glycoprotein expressed by peripheral T lymphocytes and thymocytes.
Buffer
PBS containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: PE State: Lyophilized purified IgG Label: R. Phycoerythrin (RPE)
Reconstitution Method
Restore with 1 ml distilled water
Conjugation
PE
Storage
Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE!
Stability
Shelf life: one year from despatch.
Shipping
Blue Ice
Background
CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
