Images
Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western blot analysis using CD69 monoclonal antibody, clone 8B6 (Cat # MAB10540) against, Jurkat (1), L1210 (2) and TPH-1 (3) cell lysate.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of paraffin-embedded human tonsil tissues using CD69 monoclonal antibody, clone 8B6 (Cat # MAB10540).

Flow Cytometry
Application

Flow Cytometry

Flow cytometric analysis of Jurkat cells using CD69 monoclonal antibody, clone 8B6 (Cat # MAB10540) (green) and negative control (purple).

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against partial recombinant CD69.

    Immunogen

    Recombinant protein corresponding to human CD69.

    Host

    Mouse

    Theoretical MW (kDa)

    22.5

    Reactivity

    Human

    Form

    Liquid

    Isotype

    IgG1

    Recommend Usage

    ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In ascites (0.03% sodium azide)

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Cell lysate)

    Western blot analysis using CD69 monoclonal antibody, clone 8B6 (Cat # MAB10540) against, Jurkat (1), L1210 (2) and TPH-1 (3) cell lysate.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissues using CD69 monoclonal antibody, clone 8B6 (Cat # MAB10540).

    Enzyme-linked Immunoabsorbent Assay

    Flow Cytometry

    Flow cytometric analysis of Jurkat cells using CD69 monoclonal antibody, clone 8B6 (Cat # MAB10540) (green) and negative control (purple).
  • Gene Info — CD69

    Entrez GeneID

    969

    Gene Name

    CD69

    Gene Alias

    CLEC2C

    Gene Description

    CD69 molecule

    Omim ID

    107273

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants

    Other Designations

    C-type lectin domain family 2, member C|CD69 antigen (p60, early T-cell activation antigen)

  • Interactomes
  • Diseases