CD80 (T-lymphocyte Activation Antigen CD80, Activation B7-1 Antigen, BB1, CTLA-4 Counter-receptor B7.1, B7, CD28LG, CD28LG1, LAB7) (Biotin)

Referentie C2432-01F-100ug

Formaat : 100ug

Merk : US Biological

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C2432-01F CD80 (T-lymphocyte Activation Antigen CD80, Activation B7-1 Antigen, BB1, CTLA-4 Counter-receptor B7.1, B7, CD28LG, CD28LG1, LAB7) (Biotin)

Clone Type
Polyclonal
Host
mouse
Source
human
Isotype
IgG1
Grade
Affinity Purified
Applications
E FC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Human CD80 (B7-1) is a costimulatory ligand for CD28 and CTLA-4. CD80 is expressed on activated B cells. C2432-01F blocks binding of soluble CD152 Ig fusion protein to CD80.||Applications: |Suitable for use in Flow Cytometry and ELISA. |Other applications not tested.||Recommended Dilution:|Flow Cytometry: 80ul at 5ug/ml labels 5x10e5 cells.|Optimal dilutions to be determined by the researcher.||Flow Cytometry Protocol:|1.Five x 10e5 cultured Raji cells were washed and incubated 45 minutes on ice with 80ul C2432-01F at 5ug/ml. |2. Cells were then washed twice, incubated with secondary reagent.|3. Cells were then washed three times, fixed and analyzed by FACS.|3. Cells stained positive with a mean shift of 1.81 log10 fluorescent units when compared to a Mouse IgM/FITC negative control at a similar concentration. |4. Binding was blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml C2432-01E.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 7H211|Host: mouse|Source: human|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.|Purity: Purified by Protein A affinity chromatography.|Immunogen: Human CD80 (B7-1) Ig Fusion Protein.|Specificity: Recognizes human CD80.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Human CD80 (B7-1) Ig Fusion Protein.
Form
Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CD80.
References
1. C.B. Thompson, (1995) Cell 81: 979-982. 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 682-684.