CELLDATA DNAstorm™ 2.0 MagBead FFPE DNA Extraction Kit

Referentie CD509-96

Formaat : 96preps

Merk : Biotium

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Storage Conditions

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Product Description

Formalin-fixed tissue samples are a challenge for DNA extraction, often resulting in low yields and poor performance in subsequent steps. Most existing methods rely on heat to remove crosslinks and adducts, which is only partially effective and leads to additional fragmentation of labile nucleic acids. Powered by proprietary CAT5™ technology, the DNAstorm™ 2.0 MagBead FFPE Extraction Kit enhances the removal of formaldehyde-induced damage and provides DNA with higher yield and quality and greater amplifiability. The DNAstorm™ 2.0 MagBead FFPE DNA Extraction Kit is the best solution for next-generation sequencing and other advanced applications. This kit uses magnetic beads for DNA isolation and may be adapted for multiwell plate format. For spin column-based DNA purification, see our original CELLDATA DNAstorm™ 2.0 FFPE DNA Extraction Kit.

  • Purify higher quality DNA with less fragmentation
  • Extract higher yields of amplifiable DNA compared to other methods
  • CAT5™ technology for chemical reversal of formaldehyde crosslinking
  • Milder conditions and no harsh solvents
  • Better results in downstream analysis like PCR, microarray, or next-generation sequencing
  • Magnetic bead-based DNA purification adaptable for multiwell plate formats

The DNAstorm™ 2.0 DNA  extraction kit greatly accelerates the removal of formaldehyde damage and allows recovery of higher quality nucleic acids. This results in higher yields, more intact DNA, and better results in downstream analysis like PCR, microarray, or next-generation sequencing (NGS). See the product table below for our complete line of RNAstorm™ and DNAstorm™ extraction kits for FFPE tissues.

DNA from four different FFPE tumor samples (colorectal, lung, bladder, and esophagus) was extracted using the DNAstorm™ FFPE kit along with a popular competitor’s kit. Equal amounts (500 ng) of DNA were loaded and run on a pulsed field gel. Significant improvements in average DNA size are seen using the DNAstorm™ kit. “Kit R” represents a competitive commercial DNA FFPE extraction kit.

RNAstorm™ and DNAstorm™ FFPE Extraction Kits

Product Name Catalog Number Size

CELLDATA RNAstorm™ 2.0 FFPE RNA Extraction Kit

CD506

50 preps

CELLDATA DNAstorm™ 2.0 FFPE DNA Extraction Kit

CD507

50 preps

CELLDATA DNAstorm™/RNAstorm™ 2.0 Combination Kit

CD508

50 preps

CELLDATA RNAstorm™ 2.0 MagBead FFPE RNA Extraction Kit

CD510-96

96 preps

CELLDATA DNAstorm™ 2.0 MagBead FFPE DNA Extraction Kit

CD509-96

96 preps

References

  1. Am J Surg Pathol, 43, 1720

    DOI: 10.1097/PAS.0000000000001338