D-Lactate Assay Kit, BioAssay™, Colorimetric

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection.||Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. Lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565nm, is proportionate to the lactate concentration in the sample.||Key Features:|Sensitive and accurate: The detection limit of 0.05mM and linearity up to 2mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1mM and linearity up to 1mM D-lactate in a 96-well plate assay.||Convenient: The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 minutes. Room temperature assay. No 37°C heater is needed.||High-throughput: Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.||Application:|Direct Assays: D-lactate in serum, plasma, and cell media samples.||Kit Contents: (100 tests in 96-well plates):|L1011-04-A: Assay Buffer, 1x10ml|L1011-04-B: Enzyme A, 1x120ul |L1011-04-C: Enzyme B, 1x120ul|L1011-04-D: NAD Solution, 1x1ml|L1011-04-E: MTT Solution, 1x1.5ml|L1011-04-F: Standard (20mM D-Lactate), 1x1ml||Storage and Stability:|Store components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.